Direct excitation of deep dorsal horn neurones in the rat spinal cord by the activation of postsynaptic P2X receptors.

The Journal of Physiology
Hiroaki ShiokawaMegumu Yoshimura

Abstract

ATP mediates somatosensory transmission in the spinal cord through the activation of P2X receptors. Nonetheless, the functional significance of postsynaptic P2X receptors in spinal deep dorsal horn neurones is still not yet well understood. Using the whole-cell patch-clamp technique, we investigated whether the activation of postsynaptic P2X receptors can modulate the synaptic transmission in lamina V neurones of postnatal day (P) 9-12 spinal cord slices. At a holding potential of -70 mV, ATPgammaS (100 microm), a nonhydrolysable ATP analogue, generated an inward current, which was resistant to tetrodotoxin (1 microm) in 61% of the lamina V neurones. The ATPgammaS-induced inward current was accompanied by a significant increase in the frequency of glutamatergic miniature excitatory postsynaptic currents (mEPSCs) in the majority of lamina V neurones. The ATPgammaS-induced inward current was not reproduced by P2Y receptor agonists, UTP (100 microm), UDP (100 microm), and 2-methylthio ADP (100 microm), and it was also not affected by the addition of guanosine-5'-O-(2-thiodiphosphate) (GDPbetaS) into the pipette solution, thus suggesting that ionotropic P2X receptors were activated by ATPgammaS instead of metabotropic P2Y receptors...Continue Reading

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Citations

Feb 12, 2009·Purinergic Signalling·Maria Luisa Cotrina, Maiken Nedergaard
Oct 24, 2007·Molecular Neurobiology·Kerstin WirknerPeter Illes
Jul 31, 2007·Brain Research·Naoki KodamaRyuji Matsuo
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Jan 31, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shusheng WangGuang-Yin Xu

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