Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes

PloS One
Kanako NoritakeKoichi Uemura

Abstract

Direct exposure of cardiomyocytes to ethanol causes cardiac damage such as cardiac arrythmias and apoptotic cell death. Cardiomyocytes are connected to each other through intercalated disks (ID), which are composed of a gap junction (GJ), adherens junction, and desmosome. Changes in the content as well as the subcellular localization of connexin43 (Cx43), the main component of the cardiac GJ, are reportedly involved in cardiac arrythmias and subsequent damage. Recently, the hippo-YAP signaling pathway, which links cellular physical status to cell proliferation, differentiation, and apoptosis, has been implicated in cardiac homeostasis under physiological as well as pathological conditions. This study was conducted to explore the possible involvement of junctional intercellular communication, mechanotransduction through cytoskeletal organization, and the hippo-YAP pathway in cardiac damage caused by direct exposure to ethanol. HL-1 murine atrial cardiac cells were used since these cells retain cardiac phenotypes through ID formation and subsequent synchronous contraction. Cells were exposed to 0.5-2% ethanol; significant apoptotic cell death was observed after exposure to 2% ethanol for 48 hours. A decrease in Cx43 levels was al...Continue Reading

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Citations

Feb 12, 2017·European Journal of Cell Biology·Marta Tellez-GabrielDominique Heymann
Feb 27, 2020·Nutrients·Joaquim Fernández-Solà
Sep 15, 2017·The Journal of Toxicological Sciences·Kanako NoritakeKoichi Uemura
Mar 30, 2017·Naunyn-Schmiedeberg's Archives of Pharmacology·Su TuCheng-Jian Yang

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Methods Mentioned

BETA
PCR
fluorescence microscopy
transfection
nuclear translocation
ubiquitination

Software Mentioned

PSmOrange
vimentin
ImageJ

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