Direct fermentation route for the production of acrylic acid

Metabolic Engineering
Hun Su ChuKwang Myung Cho

Abstract

There have been growing concerns regarding the limited fossil resources and global climate changes resulting from modern civilization. Currently, finding renewable alternatives to conventional petrochemical processes has become one of the major focus areas of the global chemical industry sector. Since over 4.2 million tons of acrylic acid (AA) is annually employed for the manufacture of various products via petrochemical processes, this chemical has been the target of efforts to replace the petrochemical route by ecofriendly processes. However, there has been limited success in developing an approach combining the biological production of 3-hydroxypropionic acid (3-HP) and its chemical conversion to AA. Here, we report the first direct fermentative route for producing 0.12 g/L of AA from glucose via 3-HP, 3-HP-CoA, and Acryloyl-CoA, leading to a strain of Escherichia coli capable of directly producing acrylic acid. This route was developed through extensive screening of key enzymes and designing a novel metabolic pathway for AA.

References

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Citations

Jun 27, 2019·Biotechnology for Biofuels·Christian Arenas-LópezKlaus Winzer
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Jia-Wei LiYun-Peng Bai
Nov 18, 2017·The Biochemical Journal·Selçuk AslanArren Bar-Even
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen
Jan 13, 2021·Macromolecular Rapid Communications·Hugo Fouilloux, Christophe M Thomas
Mar 9, 2021·PLoS Computational Biology·Alexandre OliveiraOscar Dias
Jul 2, 2021·Advanced Science·Céline MontanariLars A Berglund
Oct 25, 2017·Biomacromolecules·Vesna V PanicPavle M Spasojevic
Sep 14, 2021·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Yichen QiuJonathan Sperry

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