Direct flow cytometry measurements reveal a fine-tuning of symbiotic cell dynamics according to the host developmental needs in aphid symbiosis

Scientific Reports
Pierre SimonetFederica Calevro

Abstract

Endosymbiotic associations constitute a driving force in the ecological and evolutionary diversification of metazoan organisms. Little is known about whether and how symbiotic cells are coordinated according to host physiology. Here, we use the nutritional symbiosis between the insect pest, Acyrthosiphon pisum, and its obligate symbiont, Buchnera aphidicola, as a model system. We have developed a novel approach for unculturable bacteria, based on flow cytometry, and used this method to estimate the absolute numbers of symbionts at key stages of aphid life. The endosymbiont population increases exponentially throughout nymphal development, showing a growing rate which has never been characterized by indirect molecular techniques. Using histology and imaging techniques, we have shown that the endosymbiont-bearing cells (bacteriocytes) increase significantly in number and size during the nymphal development, and clustering in the insect abdomen. Once adulthood is reached and the laying period has begun, the dynamics of symbiont and host cells is reversed: the number of endosymbionts decreases progressively and the bacteriocyte structure degenerates during insect aging. In summary, these results show a coordination of the cellular ...Continue Reading

References

May 6, 1999·Journal of Molecular Evolution·K Komaki, H Ishikawa
Nov 26, 1999·International Journal of Food Microbiology·N RattanasomboonC M McFarlane
Sep 9, 2000·FEMS Microbiology Reviews·J Vives-RegoG Nebe-von Caron
Jan 28, 2003·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Toru MiuraDavid L Stern
Oct 14, 2003·PLoS Biology·Christian BraendleDavid L Stern
Apr 6, 2004·Journal of Microbiological Methods·Federica CalevroJean-Michel Fayard
Jul 17, 2008·Biology of the Cell·Gaël Le TrionnaireDenis Tagu
Jan 9, 2009·Proceedings. Biological Sciences·E Akman Gündüz, A E Douglas
Jun 17, 2009·Applied Microbiology and Biotechnology·Mateja KramerVojko Kmetec
Feb 27, 2010·PLoS Biology·UNKNOWN International Aphid Genomics Consortium
Mar 9, 2010·Current Opinion in Biotechnology·Bryan P TracyEleftherios T Papoutsakis
Jun 15, 2010·Applied and Environmental Microbiology·Mohiuddin M Taimur KhanAnne K Camper
Jun 22, 2011·Molecular Ecology·Nicole M Gerardo, Alex C C Wilson
Apr 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryuichi KogaTakema Fukatsu
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Denis VoroninMark J Taylor
Jul 28, 2012·Current Biology : CB·Jennifer J Wernegreen
Jan 3, 2013·Journal of Applied Microbiology·M Bouix, S Ghorbal
Nov 5, 2013·Applied and Environmental Microbiology·Alice M LaughtonNicole M Gerardo
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniel R G PriceAlex C C Wilson
Jan 3, 2014·Insect Science·Jean PeccoudJean-Christophe Simon
Jul 20, 2014·Microbial Ecology·Fabio Cleisto Alda DossiFernando Luis Cônsoli
Sep 23, 2014·Current Biology : CB·Aurélien VigneronAbdelaziz Heddi
Jan 1, 2012·Insects·Carolin RatzkaHeike Feldhaar
Jan 1, 2011·Insects·Kevin J Vogel, Nancy A Moran

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Citations

Nov 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rebecca A Chong, Nancy A Moran
Apr 4, 2017·Insect Science·Isabel H Skidmore, Allison K Hansen
Feb 6, 2017·Applied and Environmental Microbiology·Matthew R Doremus, Kerry M Oliver
May 18, 2018·G3 : Genes - Genomes - Genetics·Dohyup KimAllison K Hansen
Nov 22, 2019·FEMS Microbiology Letters·Sergio López-Madrigal, Elves H Duarte
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Pierre SimonetFederica Calevro
May 28, 2021·Scientific Reports·R GaetaniB Masenelli
Jun 16, 2021·The ISME Journal·Flávia P FrancoMarcio C Silva-Filho
Apr 9, 2021·G3 : Genes - Genomes - Genetics·Daniel Pers, Allison K Hansen
Aug 26, 2021·Proceedings of the National Academy of Sciences of the United States of America·Julie PerreauNancy A Moran
Nov 25, 2021·Proceedings. Biological Sciences·Mathilda WhittleSinead English

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Methods Mentioned

BETA
flow cytometry
flow
dissection
PCR

Software Mentioned

FACSDiva
nlstools R
CellF Imaging System
CellF
R R

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