Direct fluconazole susceptibility testing of positive Candida blood cultures by flow cytometry

Mycoses
Bernard RudenskyDavid Raveh

Abstract

The standard methods for yeast susceptibility testing require 24-48 h of incubation. As there has been an increase in incidence of non-albicans Candida species, the clinician is very often wary of initiating therapy with fluconazole until a final susceptibility report is generated, especially when treating very sick patients. A rapid reliable susceptibility testing method would enable the clinician to prescribe fluconazole, thus avoiding more toxic or expensive therapy. To determine the feasibility of direct susceptibility testing of Candida species to fluconazole by a rapid flow cytometric method, 50 Candida strains were seeded into blood culture bottles and were tested for susceptibility to fluconazole directly from the bottles after their being flagged as positive by the blood culture instrument. Minimal inhibitory concentration (MIC) determined by fluorescent flow cytometry (FACS) showed excellent agreement to that determined by macrodilution. Following the seeding experiments, 30 true patient specimens were tested directly from positive blood cultures, and MIC determined by both methods showed excellent agreement. Antifungal susceptibility testing by FACS directly from positive blood culture bottles is a reliable, rapid me...Continue Reading

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Citations

Jan 30, 2013·Antimicrobial Agents and Chemotherapy·Cecília Rocha da SilvaHélio Vitoriano Nobre Júnior
Jul 23, 2009·Expert Review of Anti-infective Therapy·M Anaul Kabir, Mohammad Asif Hussain
Feb 3, 2015·Revista iberoamericana de micología·Tatiane BenaducciMaria José Soares Mendes-Giannini
Jul 14, 2017·Journal of Clinical Laboratory Analysis·Yasemin Oz, Egemen Gokbolat
Apr 23, 2021·Journal of Clinical Microbiology·Sarah IsraelMaya Korem

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