Direct fluorescent glycan labeling with recombinant sialyltransferases

Glycobiology
Zhengliang WuV. Kalabokis

Abstract

Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.

References

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Citations

Jan 27, 2021·Proceedings of the National Academy of Sciences of the United States of America·Simon WisnovskyCarolyn R Bertozzi
Aug 25, 2020·Bioconjugate Chemistry·Zhengliang L WuYue Chen
Sep 8, 2020·Journal of Proteome Research·Hayden Wilkinson, Radka Saldova
Jun 9, 2021·Chembiochem : a European Journal of Chemical Biology·Eun Ju Kim
Oct 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ryoma HombuSheldon Park
Oct 16, 2021·Scientific Reports·Zhengliang L Wu, James M Ertelt
Nov 9, 2021·Bioconjugate Chemistry·Min CongMohammad Rashidian

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

DFGL

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