Direct gas chromatographic determination of dechloroethylcyclophosphamide following microsomal incubation of cyclophosphamide

Journal of Chromatography. B, Biomedical Applications
F BohnenstengelH K Kroemer

Abstract

A method for the sensitive determination of dechloroethylcylclophosphamide (3-DCl) in microsomal incubation mixtures was developed. 3-DCl, a side-chain oxidation product of cyclophosphamide (CP), was isolated by extraction with acetic acid ethyl ester following solid-phase extraction on C8 cartridges. Quantification of the metabolite was performed by direct capillary gas chromatography with a nitrogen-phosphorus detector without prior derivatization. The method showed good sensitivity and reproducibility with a detection limit of 1 ng/ml and a limit of quantification of 5 ng/ml. The suitability of the method is shown for the quantification of 3-DCl following incubation of CP with human liver microsomes.

References

Nov 18, 1992·Journal of the National Cancer Institute·A V BoddyJ R Idle
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Nov 22, 1986·Lancet·M P GorenF E Pell
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May 1, 1994·British Journal of Cancer·J M HartleyR L Souhami

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Citations

May 19, 2005·Journal of Clinical Laboratory Analysis·Frank BaumannRainer Preiss
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·A PaciP Prognon

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