Direct gene delivery of human tissue kallikrein reduces blood pressure in spontaneously hypertensive rats

The Journal of Clinical Investigation
C WangJ Chao

Abstract

Hypertension is a multigene and multifactorial disorder affecting approximately 25% of the population. To demonstrate potential therapeutic effects of human tissue kallikrein in hypertension, spontaneously hypertensive rats were subjected to somatic gene therapy. Two human tissue kallikrein DNA constructs, one under the promoter control of the metallothionein metal response element and the other under the control of the Rous sarcoma virus 3'-LTR, were generated. We delivered naked DNA constructs into spontaneously hypertensive rats via intravenous injection. The expression of human tissue kallikrein in rats was identified in the heart, lung, and kidney by reverse transcription polymerase chain reaction followed by Southern blot analysis and an ELISA specific for human tissue kallikrein. A single injection of both human kallikrein plasmid DNA constructs caused a sustained reduction of blood pressure which began 1 wk after injection and continued for 6 wk. A maximal effect of blood pressure reduction of 46 mmHg in rats was observed 2-3 wk after injection with kallikrein DNA as compared to rats with vector DNA (n = 6, P < 0.05). The hypotensive effect caused by somatic gene delivery of human tissue kallikrein in hypertensive rats ...Continue Reading

References

Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Mar 1, 1979·Japanese Circulation Journal·K ShimamotoM Miyahara
Jun 1, 1975·Circulation Research·R G GellerH R Keiser
May 1, 1992·The Journal of Clinical Investigation·A A JaffaR K Mayfield
Oct 1, 1992·British Journal of Pharmacology·T Griesbacher, F Lembeck
Mar 23, 1990·Science·J A WolffP L Felgner
Aug 1, 1990·Circulation Research·G M SaedA G Scicli
Jan 1, 1989·Annual Review of Pharmacology and Toxicology·H S Margolius
Dec 31, 1985·Biochemistry·D FukushimaS Nakanishi
Jan 1, 1986·Kidney International·A G Scicli, O A Carretero
Oct 15, 1987·Thrombosis Research·D A JohnsonA J Barrett
Nov 13, 1971·Lancet·H S MargolisA Sjoerdsma
Mar 1, 1972·Circulation Research·H S MargoliusA Sjoerdsma
Oct 1, 1980·The Journal of Clinical Endocrinology and Metabolism·K ShimamotoH S Margolius
Jul 1, 1982·The Tohoku Journal of Experimental Medicine·K ShimamotoH S Margolius
Jul 16, 1981·Nature·N GlanvilleR D Palmiter
Jun 1, 1994·Hypertension·H NollyA G Scicli
Feb 1, 1993·Journal of Bioenergetics and Biomembranes·T R Flotte

❮ Previous
Next ❯

Citations

Sep 12, 2000·Current Hypertension Reports·Y ChuD D Heistad
Jan 16, 2002·Current Hypertension Reports·Beverly L MetcalfeMichael J Katovich
Jul 24, 2010·Journal of Physiology and Biochemistry·Mohamed A HaidaraEsma R Isenovic
Oct 12, 2010·Journal of Physiology and Biochemistry·Mohamed D MorsyOsama M Osman
Jan 25, 2013·Journal of Physiology and Biochemistry·Mohamed D Morsy, Salah O Bashir
Jun 1, 1996·Immunopharmacology·B A Schölkens
Jun 1, 1997·Immunopharmacology·J Chao, L Chao
Nov 1, 1997·Trends in Cardiovascular Medicine·J Chao, L Chao
Mar 29, 2007·Nature Clinical Practice. Nephrology·Paolo MadedduSamir El-Dahr
Feb 28, 2002·Human Gene Therapy·Bruce D KlugherzRobert J Levy
Nov 14, 2003·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Paul N Hopkins, Steven C Hunt
Dec 9, 1998·American Journal of Respiratory Cell and Molecular Biology·K NarumiR G Crystal
Apr 1, 1995·The Journal of Clinical Investigation·J A Martinez, D T O'Connor
May 15, 2010·Diabetology & Metabolic Syndrome·Mohamed D MorsySherif I Zalat
Feb 8, 2014·Canadian Journal of Physiology and Pharmacology·Hussein F SakrMohamed D Morsy
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M KriegH L Davis
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·P MenetonF Alhenc-Gelas
Jul 22, 2008·Canadian Journal of Physiology and Pharmacology·Agata L GavaSilvana S Meyrelles
Nov 1, 1996·Clinical and Experimental Hypertension : CHE·Q SongL Chao
May 1, 1996·Kidney International·P MadedduV Anania
Jan 18, 2005·Experimental Physiology·Julie Chao, Lee Chao
Aug 2, 2005·Biochimica Et Biophysica Acta·Qingyu WuGary Gang Deng
Mar 1, 1997·Obstetrics and Gynecology Clinics of North America·Y YaronM I Evans
Oct 20, 1995·The Journal of Biological Chemistry·Y LiuR Debs
Jul 18, 2002·The Journal of Biological Chemistry·Visith ThongboonkerdJon B Klein
Dec 19, 2002·The Journal of Experimental Zoology·Mario ValenzuelaPedro Esponda
Dec 14, 2005·Hypertension·Mohan K Raizada, Shant Der Sarkissian

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.