Direct Generation of Human Cortical Organoids from Primary Cells

Stem Cells and Development
Monique SchukkingAlysson R Muotri

Abstract

The study of variations in human neurodevelopment and cognition is limited by the availability of experimental models. While animal models only partially recapitulate the human brain development, genetics, and heterogeneity, human-induced pluripotent stem cells can provide an attractive experimental alternative. However, cellular reprogramming and further differentiation techniques are costly and time-consuming and therefore, studies using this approach are often limited to a small number of samples. In this study, we describe a rapid and cost-effective method to reprogram somatic cells and the direct generation of cortical organoids in a 96-well format. Our data are a proof-of-principle that a large cohort of samples can be generated for experimental assessment of the human neural development.

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Citations

Dec 24, 2018·Journal of Medical Ethics·Andrea Lavazza
Dec 18, 2020·Frontiers in Neuroscience·Wei WuGabriel G Haddad
Jul 3, 2021·Advanced Functional Materials·Michael L LovettDavid L Kaplan

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Methods Mentioned

BETA
biopsy
PCR

Software Mentioned

Prism
GraphPad

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