Direct glucose production from lignocellulose using Clostridium thermocellum cultures supplemented with a thermostable β-glucosidase

Biotechnology for Biofuels
Panida PrawitwongAkihiko Kosugi

Abstract

Cellulases continue to be one of the major costs associated with the lignocellulose hydrolysis process. Clostridium thermocellum is an anaerobic, thermophilic, cellulolytic bacterium that produces cellulosomes capable of efficiently degrading plant cell walls. The end-product cellobiose, however, inhibits degradation. To maximize the cellulolytic ability of C. thermocellum, it is important to eliminate this end-product inhibition. This work describes a system for biological saccharification that leads to glucose production following hydrolysis of lignocellulosic biomass. C. thermocellum cultures supplemented with thermostable beta-glucosidases make up this system. This approach does not require any supplementation with cellulases and hemicellulases. When C. thermocellum strain S14 was cultured with a Thermoanaerobacter brockii beta-glucosidase (CglT with activity 30 U/g cellulose) in medium containing 100 g/L cellulose (617 mM initial glucose equivalents), we observed not only high degradation of cellulose, but also accumulation of 426 mM glucose in the culture broth. In contrast, cultures without CglT, or with less thermostable beta-glucosidases, did not efficiently hydrolyze cellulose and accumulated high levels of glucose. G...Continue Reading

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Citations

Nov 8, 2014·Biotechnology for Biofuels·Evert K HolwerdaLee R Lynd
Sep 1, 2015·Bioresource Technology·Harifara Rabemanolontsoa, Shiro Saka
Jun 24, 2017·Biotechnology and Applied Biochemistry·Anderson Albino GomesMaria de Lourdes Borba Magalhães
May 14, 2019·Applied Microbiology and Biotechnology·Volkmar Passoth, Mats Sandgren
Aug 14, 2019·Biotechnology and Applied Biochemistry·Bin-Bin Hu, Ming-Jun Zhu
Jul 14, 2019·Scientific Reports·S N NguyenR Berlemont
Feb 21, 2021·Applied and Environmental Microbiology·Johannes YayoAntonius J A van Maris

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Methods Mentioned

BETA
PCR

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