Direct hybridization and amplification applications for the diagnosis of infectious diseases

Journal of Clinical Laboratory Analysis
E A Wagar

Abstract

We are entering an exciting new era of molecular diagnostics in the clinical microbiology laboratory. A number of perspectives are presented in this review. First presented was a discussion of molecular diagnostics for detection of the bacterium, Chlamydia trachomatis. This is especially relevant since the tests available for this organism represent the forefront of commercial systems. Also, these tests exemplify the difficulties and advantages inherent to future molecular diagnostics for all types of disease processes. Next, a discussion of the techniques thus far employed in the field of clinical microbiology is presented. Obvious overlap exists with other areas of molecular pathology. However, the emphasis is on which techniques have proven most useful in identifying infectious agents. Finally, the features of a successful clinical microbiology diagnostics laboratory are presented, including test component requirements, laboratory personnel, quality assurance techniques, and physical laboratory setting. It is hoped that helpful advice and references are provided that will assist individual clinical laboratories as they enter the field of molecular diagnostics of infectious diseases.

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Citations

May 8, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G W Procop, M Wilson
Oct 12, 2000·Clinical Microbiology Reviews·E M ElnifroP E Klapper
Apr 27, 1999·The British Journal of Ophthalmology·E M ElnifroA B Tullo
Dec 29, 2005·Virology·Fernando CoboAngel Concha

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