Direct immobilization of biotin on the micro-patterned PEN foil treated by excimer laser

Colloids and Surfaces. B, Biointerfaces
Marcel ŠtofikPetr Slepička

Abstract

Polymers with functionalized surfaces have attracted a lot of attention in the last few years. Due to the progress in the techniques of polymer micro-patterning, miniaturized bioanalytical assays and biocompatible devices can be developed. In the presented work, we performed surface modification of polyethylene naphthalate (PEN) foil by an excimer laser beam through a photolithographic contact mask. The aim was to fabricate micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. It was found out that depending on the properties of the laser scans, a polymer surface exhibits different degrees of modification and as a consequence, different degrees of surface biotinylation can be achieved. Several affinity tests with optical detection of fluorescently labeled streptavidin were successfully performed on biotinylated micro-patterns of a PEN foil. The polymer surface properties were also evaluated by electrokinetic analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The results have shown that PEN foils can be considered suitable substrates for construction of micro-patterned bioanalytical affinity ...Continue Reading

References

Aug 9, 2007·Colloids and Surfaces. B, Biointerfaces·Zuwei MaChangyou Gao
Apr 7, 2010·Lab on a Chip·Maximilian FockeFelix von Stetten
Aug 28, 2014·Lab on a Chip·Gungun LinOliver G Schmidt

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