Direct Immunoassay for Facile and Sensitive Detection of Small Molecule Aflatoxin B1 based on Nanobody

Chemistry : a European Journal
Deng PanYanfei Shen

Abstract

Aflatoxin B1 (AFB1 ), one of the most toxic mycotoxins, is classified as a group I carcinogen and ubiquitous in various foods and agriproducts. Thus, accurate and sensitive determination of AFB1 is of great significance to meet the criteria of food safety. Direct detection of AFB1 is difficult by monoclonal antibody (mAb) with large molecular size (≈150 kD) since the target is too small to produce a detectable signal change. Herein, by combining the electrochemical properties of nanomaterials and the advantages of nanobodies, we developed a direct, highly selective and sensitive electrochemical immunosensor for small molecule detection. The proposed immunosensor had a wide calibration range of 0.01 to 100 ng mL-1 and a low detection limit of 3.3 pg mL-1 (S/N=3). Compared with the immunosensor prepared with mAb which was applied in the typical indirect immunoassay, the immunosensor in this work possessed two orders of magnitudes wider linear range and 10-fold more sensitivity. The as-obtained immunosensor was further successfully applied for sensing AFB1 in real samples. This proposed assay would provide a simple, highly sensitive and selective approach for the direct immunoassay of small molecule AFB1 , and is extendable to the...Continue Reading

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Citations

Feb 9, 2019·Analytical and Bioanalytical Chemistry·J-Pablo SalvadorM-Pilar Marco
Dec 28, 2019·Materials·Bhargav D Mansuriya, Zeynep Altintas
Feb 23, 2020·Sensors·Bhargav D Mansuriya, Zeynep Altintas
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Valerii MyndrulIgor Iatsunskyi
Oct 30, 2020·Bioelectrochemistry·Avinash Kumar SinghPratima R Solanki
Oct 16, 2020·International Journal of Biological Macromolecules·Erika A Bastos-SoaresCarla Freire C Fernandes
Aug 14, 2020·Journal of Agricultural and Food Chemistry·Yong ZhangRuijie Deng
Sep 30, 2021·Analytical Chemistry·Stanislav TrashinKarolien De Wael
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Weizheng WangSundaram Gunasekaran

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