PMID: 9558304May 19, 1998Paper

Direct immunofluorescence diagnosis of pemphigus without biopsy

Journal of Cutaneous Medicine and Surgery
C FelicianiP Amerio

Abstract

Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis. The study was to determine whether DIF is reliable on epidermal sheets separated using a suction apparatus. Thirteen patients were selected for this study: (nine with pemphigus vulgaris (PV), one with paraneoplastic pemphigus (PP), and three with pemphigus erythematosus (PE). Frozen epidermal sheets, separated from the dermis with a scalpel, were used as a substrate. Diagnosis with routine fluorescein isothiocyanate (FITC) antibodies was made. In all patients a pericellular deposition of IgG was evident and in eight of these patients a pericellular deposition of C3 was present. In two cases of PE and one of PP, the C3 deposits were also present in the lower part of basal keratinocytes. This diagnostic method without skin biopsy is easy to perform and, together with the histology and clinical aspects, could be a useful tool in the diagnosis of pemphigus. We recommend this method when the patient is allergic to local anaesthetics, the patient easily produces hypertrophic scars, or in follow...Continue Reading

References

Jun 1, 1991·Journal of the American Academy of Dermatology·N Domloge-HultschK B Yancey
Aug 1, 1983·The Journal of Investigative Dermatology·D WoodleyE Qwarnstrom

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Citations

Jun 21, 2007·Diagnostic Cytopathology·Vijay AithalElizabeth Jayaseelan

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