Direct immunofluorescence of detached gingival epithelium for diagnosis of cicatricial pemphigoid. Report of five cases

Oral Surgery, Oral Medicine, and Oral Pathology
M A Siegel, G J Anhalt

Abstract

The diagnosis of mucosal cicatricial pemphigoid is often problematic because of frequent false negative or equivocal biopsy results. We describe an immunofluorescent technique that uses detached gingival epithelium for diagnostic purposes when oral manifestations of cicatricial pemphigoid are suspected. Erythematous mucosa that is gently rubbed produces detached epithelium that is then submitted for direct immunofluorescence studies along with tissue acquired via conventional techniques. In the five cases reported, the detached gingival epithelium stained intensely for immunoglobulins and complement on the basilar pole of the basal epithelial cells. The donor sites healed without a resultant periodontal defect. This adjunctive biopsy technique offers the advantage of easily procuring gingival tissue with deposition of immunoreactants that are quite specific for the diagnosis of cicatricial pemphigoid.

References

Jul 1, 1979·Journal of the American Academy of Dermatology·W F Lever
Dec 1, 1991·Oral Surgery, Oral Medicine, and Oral Pathology·M A Siegel
Mar 1, 1989·Archives of Ophthalmology·M ZierhutG Schaumburg-Lever
Nov 1, 1989·Archives of Ophthalmology·P J McDonnellR A Eady
Jan 1, 1988·The British Journal of Dermatology·V A VenningF Wojnarowska
Jan 1, 1986·International Journal of Dermatology·H N LiuR S Rogers
Mar 1, 1986·Oral Surgery, Oral Medicine, and Oral Pathology·S SilvermanA Liu
Dec 1, 1986·International Journal of Dermatology·T P Chorzelski, S Jablońska
Feb 1, 1987·Journal of the American Academy of Dermatology·P G Lang, M J Tapert
Mar 1, 1985·Oral Surgery, Oral Medicine, and Oral Pathology·T B AufdemorteS M Parel
Oct 1, 1974·Archives of Dermatology·S F Bean
Mar 1, 1972·Oral Surgery, Oral Medicine, and Oral Pathology·J F Hasler
Apr 1, 1971·Archives of Otolaryngology·G Shklar, P L McCarthy
Oct 1, 1983·American Journal of Ophthalmology·B J Mondino, S I Brown
Mar 1, 1984·The British Journal of Dermatology·J N LeonardL Fry
Dec 1, 1982·Journal of the American Academy of Dermatology·R S RogersS H Nightingale
Apr 1, 1980·The British Journal of Dermatology·R M Fine, D R Weathers
Jan 1, 1981·Oral Surgery, Oral Medicine, and Oral Pathology·T E Daniels, C Quadra-White
Jan 1, 1981·Oral Surgery, Oral Medicine, and Oral Pathology·G Laskaris, A Angelopoulos
Sep 1, 1981·Journal of Periodontology·R J Nisengard, M Neiders

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Citations

Jan 24, 2002·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Richard C K JordanJoseph A Regezi
Mar 7, 2014·Journal of Periodontology·Hiroyasu EndoTakanori Ito
Sep 7, 2007·The British Journal of Oral & Maxillofacial Surgery·Crispian Scully, Lorenzo Lo Muzio
Jun 30, 2005·Oral Diseases·J BaganC Scully
Feb 22, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Taihei HayakawaTakashi Hashimoto
Apr 1, 1996·Dermatologic Clinics·S L Zunt
Apr 26, 2019·The British Journal of Dermatology·B CareyJ Setterfield
Sep 27, 2000·Journal of the American Academy of Dermatology·T E Fleming, N J Korman
Oct 16, 1999·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·S DayanA R Ahmed

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