Direct Immunofluorescence Using Non-Lesional Buccal Mucosa in Mucous Membrane Pemphigoid

Frontiers in Medicine
M KamaguchiH Shimizu

Abstract

Mucous membrane pemphigoid (MMP) is a rare organ-specific autoimmune subepithelial blistering disease with predominantly mucosal erosions, most frequently affecting the gingiva. Erosions in the oral cavity usually result in markedly decreased quality of life. The major autoantigens are BP180 and laminin332, which are components of basement membrane proteins in the skin and mucosa. Diagnosis is usually difficult due to histological destruction of the tissue and low autoantibody titers. In this study, we evaluated the diagnostic value of direct immunofluorescence (DIF) using non-lesional buccal mucosa in seven cases of MMP. In all seven patients, gingival lesions were clinically observed, and in one of the seven patients, buccal lesions were also clinically observed. First, we performed DIF to detect tissue-bound autoantibodies and complement. DIF from non-lesional buccal mucosa revealed linear deposits of IgG and C3 at the basement membrane zone in all cases. To detect autoantibodies, indirect immunofluorescence (IIF), BP180-NC16A ELISA and immunoblotting were performed. Surprisingly, circulating autoantibodies were unable to be detected in any of the cases by ELISA, IIF, or immunoblotting. Furthermore, histological separation w...Continue Reading

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Citations

Dec 24, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Ciro GilvettiAndrew William Barrett
Feb 21, 2019·Current Protocols in Pharmacology·Anika KasprickRalf J Ludwig
Jan 29, 2020·The American Journal of Dermatopathology·Randie H Kim, Nooshin K Brinster
Jul 18, 2019·American Journal of Clinical Dermatology·Hanan RashidBarbara Horváth
Jul 30, 2020·Oral Diseases·Massimo PetruzziFedora Della Vella
Feb 12, 2019·Frontiers in Immunology·Mayumi Kamaguchi, Hiroaki Iwata
Aug 10, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mayumi KamaguchiHiroshi Shimizu
Sep 26, 2019·Frontiers in Immunology·Sandra SaschenbreckerWolfgang Schlumberger
Mar 7, 2021·Journal of Clinical Medicine·Stefania LeuciMichele Davide Mignogna
Mar 9, 2021·Journal of the American Academy of Dermatology·Carmen M MontagnonJulia S Lehman

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Methods Mentioned

BETA
ELISA
biopsy
biopsying

Software Mentioned

Graph Pad PRISM

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