Direct immunofluorescent detection of Legionella pneumophila in respiratory specimens.

Journal of Clinical Microbiology
W C WinnC V Broome

Abstract

Respiratory secretions from patients with clinically suspected Legionnaires pneumonia were examined by direc immunofluorescent tests at the Medical Center Hospital of Vermont and at the Center for Disease Control. No fluorescent bacteria were found by either laboratory in eight specimens from eight patients who were seronegative. Twenty specimens were obtained from seven patients who had serologically confirmed Legionnaires disease. Four of the seven cases were identified at the Medical Center Hospital of Vermont, and six of the seven were identified at the Center for Disease Control. Of 20 specimens, 8 were positive at the Center for Disease Control (six or more bacilli per slide), and 7 specimens were suspicious (one to five bacilli per slide); at the Medical Center Hospital of Vermont, 4 of 20 specimens were positive, and 2 were suspicious. The inclusion of a rhodamine-conjugated counterstain at the Center for Disease Control facilitated the examination and may have improved the sensitivity. Smears from transtracheal aspirates, bronchoscopic aspirates, transcutaneous lung aspirates, pleural fluids, and tracheal aspirate-expectorated sputum produced positive results. Several specimens contained fluorescing bacilli when staine...Continue Reading

References

Sep 1, 1978·Journal of Clinical Microbiology·J C FeeleyH W Smith
Dec 2, 1978·Lancet·P H EdelsteinS M Finegold
Apr 1, 1979·Annals of Internal Medicine·R M McKinneyB M Thomason
Dec 1, 1977·The New England Journal of Medicine·D W FraserP S Brachman
Sep 1, 1978·Journal of Clinical Microbiology·W B CherryR E Weaver
Jan 1, 1979·Annals of Internal Medicine·C V BroomeB R MacPherson
Jan 1, 1979·Journal of Clinical Microbiology·R M McKinneyC W Moss
Apr 1, 1979·Annals of Internal Medicine·D W GumpW B Cherry
Sep 1, 1978·Annals of Internal Medicine·B D KirbyS M Finegold

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