Direct in vivo monitoring of sarcoplasmic reticulum Ca2+ and cytosolic cAMP dynamics in mouse skeletal muscle.

The Journal of Cell Biology
Rüdiger RudolfT Pozzan

Abstract

Skeletal muscle contraction depends on the release of Ca(2+) from the sarcoplasmic reticulum (SR), but the dynamics of the SR free Ca(2+) concentration ([Ca(2+)](SR)), its modulation by physiological stimuli such as catecholamines, and the concomitant changes in cAMP handling have never been directly determined. We used two-photon microscopy imaging of GFP-based probes expressed in mouse skeletal muscles to monitor, for the first time in a live animal, the dynamics of [Ca(2+)](SR) and cAMP. Our data, which were obtained in highly physiological conditions, suggest that free [Ca(2+)](SR) decreases by approximately 50 microM during single twitches elicited through nerve stimulation. We also demonstrate that cAMP levels rise upon beta-adrenergic stimulation, leading to an increased efficacy of the Ca(2+) release/reuptake cycle during motor nerve stimulation.

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Citations

Apr 8, 2010·Histochemistry and Cell Biology·Roberto WeigertAndrius Masedunskas
Jan 14, 2010·Pflügers Archiv : European journal of physiology·Ramón Jiménez-MorenoOsvaldo Delbono
Jan 18, 2012·Pflügers Archiv : European journal of physiology·Zhong-Min WangOsvaldo Delbono
Jan 1, 2008·Nature Methods·Debbie Willoughby, Dermot M F Cooper
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ira V RöderRüdiger Rudolf
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christina ValkovaChristoph Kaether
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Valentina LissandronTullio Pozzan
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·M CanatoG J M Stienen
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ellen AbellMary N Teruel
Sep 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shen TangJenny J Yang
Jun 27, 2013·The Journal of Cell Biology·Roberto WeigertPanomwat Amornphimoltham
Mar 19, 2008·The Journal of General Physiology·Eduardo RíosMichael D Stern
Aug 18, 2010·The Journal of General Physiology·Leandro RoyerEduardo Ríos
Jul 27, 2011·The Journal of General Physiology·Monika SztretyeEduardo Ríos
May 1, 2013·The Journal of General Physiology·Werner Melzer
Jan 22, 2010·Molecular Biology of the Cell·Marvin BentleyJesse C Hay
May 9, 2014·Journal of Cell Science·Andras T DeakRoland Malli
Jun 14, 2014·Nature Communications·Junji SuzukiMasamitsu Iino
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sandrine PouvreauJingsong Zhou
Aug 13, 2014·European Journal of Applied Physiology·Morten HostrupVibeke Backer
Oct 5, 2011·Cellular and Molecular Life Sciences : CMLS·Enrico Zampese, Paola Pizzo
Feb 9, 2012·The Journal of Physiology·Lourdes FigueroaEduardo Ríos
Mar 16, 2016·Metabolism: Clinical and Experimental·Dong-Xia ZhangYue-Sheng Huang
May 1, 2009·The Journal of Physiology·Leandro Royer, Eduardo Ríos
Mar 24, 2007·The Journal of Physiology·Gonzalo Pizarro
Feb 15, 2011·Cell Calcium·Paola PizzoTullio Pozzan
Oct 12, 2010·Advanced Drug Delivery Reviews·Panomwat AmornphimolthamRoberto Weigert
Mar 6, 2009·Cellular Signalling·Ira Verena RöderRüdiger Rudolf
Jul 22, 2015·Medicine and Science in Sports and Exercise·Anders KalsenJens Bangsbo
Apr 20, 2010·The EMBO Journal·Vanina RomanelloMarco Sandri
Dec 7, 2013·Acta Crystallographica. Section D, Biological Crystallography·Ying ZhangIrene T Weber
Dec 23, 2008·Biochimica Et Biophysica Acta·Tullio Pozzan, Rüdiger Rudolf

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Methods Mentioned

BETA
x-ray microanalysis
confocal microscopy
Fluorescence

Software Mentioned

ImageJ
Excel

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