Direct incorporation of mesenchymal stem cells into a Nanofiber scaffold - in vitro and in vivo analysis.

Scientific Reports
Karl F SchüttlerJürgen R J Paletta

Abstract

Bony defects are a common problem in musculoskeletal surgery. Replacement with autologous bone grafts is limited by availability of transplant material. Sterilized cancellous bone, while being osteoconductive, has limited osteoinductivity. Nanofiber scaffolds are currently used for several purposes due to their capability of imitating the extracellular matrix. Furthermore, they allow modification to provide functional properties. Previously we showed that electrospun nanofiber scaffolds can be used for bone tissue regeneration. While aiming to use the osteoinductive capacities of collagen type-I nanofibers we saw reduced scaffold pore sizes that limited cellular migration and thus colonization of the scaffolds. Aim of the present study was the incorporation of mesenchymal stem cells into the electrospinning process of a nanofiber scaffold to produce cell-seeded nanofiber scaffolds for bone replacement. After construction of a suitable spinning apparatus for simultaneous electrospinning and spraying with independently controllable spinning and spraying devices and extensive optimization of the spinning process, in vitro and in vivo evaluation of the resulting scaffolds was conducted. Stem cells isolated from rat femora were inco...Continue Reading

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Citations

Mar 31, 2021·Npj Regenerative Medicine·Andrea De PieriDimitrios I Zeugolis

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Methods Mentioned

BETA
light microscopy
scanning electron microscopy

Software Mentioned

Qwin
QUIPS
Blue Hill®
SPSS

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