Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling

Analytica Chimica Acta
Jiapeng LengYinlong Guo

Abstract

A rapid method for fatty acids (FAs) comparative profiling based on carboxyl-specific stable isotope labeling (SIL) and direct infusion electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS) is established. The design of the method takes advantage of the three-dimensional characteristics of IM-MS including drift time, m/z and ion intensity, for comparison of d0-/d6-2,4-dimethoxy-6-piperazin-1-yl pyrimidine (DMPP)-labeled FAs. In particular, without chromatographic separation, the method allowed direct FAs profiling in complex samples due to the advantageous priority of DMPP in signal enhancement as well as the extra resolution that IM-MS offered. Additionally, the d0-/d6-DMPP-labeled FAs showed expected features, including very similar drift times, 6 Da mass deviations, specific reporter ions, similar MS responses, and adherence to the drift time rule regarding the influence of carbon chain length and unsaturation on relative drift times. Therefore, the introduction of isotope analogs minimized the matrix effect and variations in quantification and ensured accurate identification of non-targeted FAs by those typical features. Peak intensity ratios between d0-/d6-DMPP-labeled ions were subsequently used in relative q...Continue Reading

References

Jun 13, 1998·Analytical Chemistry·C S HoaglundD E Clemmer
Jul 13, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·Y J YangB C Chung
Nov 15, 2000·Cellular and Molecular Life Sciences : CMLS·A K Dutta-Roy
Jun 24, 2003·Mass Spectrometry Reviews·William J Griffiths
Sep 21, 2007·Nature Reviews. Cancer·Javier A Menendez, Ruth Lupu
Jul 5, 2008·Nature Protocols·Brandon T RuotoloCarol V Robinson
Oct 22, 2008·Journal of the American Society for Mass Spectrometry·Isabel Riba GarciaSimon J Gaskell
Apr 27, 2010·Chemical Society Reviews·Charlotte UetrechtAlbert J R Heck
Aug 14, 2010·Analytical Chemistry·James G BollingerMichael H Gelb
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Alexandre A ShvartsburgThomas O Metz
Feb 21, 2013·Journal of the American Society for Mass Spectrometry·Eric D MerkleyJoshua N Adkins
Jul 23, 2013·Journal of Pharmaceutical and Biomedical Analysis·Jiapeng LengYinlong Guo
Mar 5, 2014·Rapid Communications in Mass Spectrometry : RCM·Hongli LiHerbert H Hill
Mar 22, 2014·Analytical and Bioanalytical Chemistry·André M MuradElibio L Rech

❮ Previous
Next ❯

Citations

Nov 2, 2016·Analytical Chemistry·Jakub PřichystalVladimír Havlíček
Mar 17, 2017·Nature Protocols·Giuseppe Paglia, Giuseppe Astarita
Oct 13, 2017·Journal of Separation Science·Valentina D'AtriDavy Guillarme
Nov 22, 2018·Analytical and Bioanalytical Chemistry·Jörg SchlotterbeckMichael Lämmerhofer
Feb 10, 2021·Critical Reviews in Analytical Chemistry·Vladimir G Zaikin, Roman S Borisov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.