Direct inhibition of voltage-dependent Ca(2+) fluxes by ethanol and higher alcohols in rabbit T-tubule membranes

European Journal of Pharmacology
M OzS M Dunn

Abstract

The effects of ethanol and higher alcohols on 45Ca(2+) fluxes, mediated by voltage-dependent Ca(2+) channels (VDCCs), were investigated in inside-out transverse (T)-tubule membrane vesicles from rabbit skeletal muscle. 45Ca(2+) effluxes were induced by membrane potentials generated via establishing K(+) gradients across the vesicles, and were significantly inhibited by the inorganic Ca(2+) channel blocker La(3+) (1 mM) and the Ca(2+) channel antagonist nifedipine (1-10 microM). Ethanol, in the concentration range of 100-400 mM, caused a significant suppression of depolarization-induced 45Ca(2+) fluxes. Ethanol also functionally modulated the effect of nifedipine (1-10 microM) and the Ca(2+) channel agonist Bay K 8644 (1 microM) on Ca(2+) effluxes. Pretreatment with pertussis toxin (5 microg/ml) or phorbol 12-myrstate 13-acetate (PMA, 50 nM) did not affect the ethanol inhibition of 45Ca(2+) fluxes. Further experiments with alcohols revealed that butanol, hexanol, octanol and decanol also significantly inhibited 45Ca(2+) effluxes. However, undecanol and dodecanol did not cause any significant change on 45Ca(2+) fluxes, indicating that the effects of alcohols on 45Ca(2+) effluxes exhibit a cut-off phenomenon. In radioligand bindin...Continue Reading

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Citations

Feb 1, 2003·Journal of Biochemical and Biophysical Methods·Murat OzMeral Dinc
Sep 15, 2009·Acta Pharmacologica Sinica·Naciye Yaktubay DöndaşErgin Singirik
Jun 8, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Bassem SadekMurat Oz
Dec 9, 2003·The Journal of Physiology·Roberta SqueccoFabio Francini
Feb 8, 2002·Archives of Biochemistry and Biophysics·Murat OzSusan M J Dunn

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