Direct inhibitory effect of uremic toxins and polyamines on proliferation of VERO culture cells

Experimental and Molecular Pathology
G StabelliniC Calastrini

Abstract

The dialysate fluid of uremic patients exhibits, in vitro, an inhibitory effect on cell growth, owing to urea, guanidino compounds, and substances named middle molecules. The polyamines are compounds which exhibit high levels in biological fluids during either normal development or disease such as psoriasis, uremia, and tumors. Dialysate and middle molecules show toxicity and degeneration of the organotype cultures, whereas the free polyamines and nonrecirculated dialysate do not have any toxic effect. The aim of this study is to analyze the effects of polyamines, nonrecirculated dialysate, and middle molecules of uremic patients in periodic hemodialysis on cultured VERO (fibroblast-like cells) growth. These cells show an inhibition of growth in middle molecules or 2 x 10(-4) M putrescine and a stimulation with nonrecirculated dialysate and 2 x 10(-8) M putrescine. The effect is different because the cultures with middle molecules begin growth again after 24 hr, whereas in the presence of 2 x 10(-4) M putrescine no further growth is observed. Cells maintained in middle molecules + 2 x 10(-8) M putrescine show an irreversible degeneration, attesting a toxic effect due to the low molarities of putrescine. The electron microscopy ...Continue Reading

References

Aug 1, 1991·Experimental Cell Research·B BrüneS Orrenius
Jul 1, 1991·Kidney International·A P WieslanderU C Boberg
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
May 1, 1980·Artificial Organs·N K ManJ L Funck-Brentano

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Citations

Jul 3, 2013·Pflügers Archiv : European journal of physiology·Carolien M S SchophuizenRosalinde Masereeuw
Jun 7, 2005·Seminars in Dialysis·Asuman YavuzClaudio Ronco
Jul 5, 2006·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Katsunori YoshidaYoshihiko Hirao
Mar 15, 2018·American Journal of Physiology. Renal Physiology·James G AthertonErnő Lindner
Dec 19, 2003·Advances in Renal Replacement Therapy·Raymond Camille VanholderPeter Paul De Deyn

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