Direct injection analysis of chlorzoxazone and its major metabolite 6-hydroxychlorzoxazone in human serum using a semipermeable surface (SPS) HPLC column

Biomedical Chromatography : BMC
A Haque, J T Stewart


A semipermeable surface (SPS) HPLC column has been used to develop a direct injection method for the simultaneous determination of chlorzoxazone and its 6-hydroxy metabolite in serum. Spiked serum was filtered and 20 microL injected directly onto the SPS column. The separation was achieved within 25 min using a mobile phase of 20:80 v/v acetonitrile-aqueous 0.05 M phosphate buffer pH 7 with detection at 230 nm. The method was linear in the concentration range 0.5-20 micrograms/mL (r > 0.999, n = 6) with an RSD < 5%. The limits of quantitation and detection were 0.5 microgram/mL and 0.1 microgram/mL (S/N > 3), respectively, for both drug and metabolite. Intraday and interday reproducibility was < or = 6% with recoveries of drug and metabolite > 94%.


Jul 1, 1979·Journal of Pharmaceutical Sciences·J T Stewart, C W Chan
May 17, 1991·Journal of Chromatography·C P DesiletsF E Regnier
Nov 1, 1990·Chemical Research in Toxicology·R PeterC S Yang
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·S H Wong
Sep 1, 1983·Journal of Pharmaceutical Sciences·R K DesirajuK T Ng
Dec 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·B J GurleyD Skelton

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