Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2

FEBS Letters
Hye-Won ParkHyewhon Rhim

Abstract

Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

References

May 19, 2006·The European Journal of Neuroscience·Hua ZhangIlya Bezprozvanny
Oct 21, 2006·Biochemical Society Transactions·J StriessnigN Singewald
Jan 11, 2008·The European Journal of Neuroscience·Rachel D GrothPaul G Mermelstein

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Citations

Jan 14, 2012·Cell Structure and Function·Zdenka Drastichova, Jiri Novotny
Mar 31, 2017·Pharmacology Research & Perspectives·Emmanuel SturchlerPatricia McDonald
Sep 2, 2017·Scientific Reports·Tamara HermosillaDiego Varela
Sep 1, 2017·Annals of Neurology·Yongjin YooMurim Choi
May 23, 2019·Cell Proliferation·Yi-Zhou TanBei Li
Apr 3, 2021·Neuropharmacology·Alessandra PorcuM Paola Castelli

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