Direct interaction and reciprocal regulation between ASK1 and calcineurin-NFAT control cardiomyocyte death and growth.

Molecular and Cellular Biology
Qinghang LiuJeffery D Molkentin

Abstract

The calcium-calmodulin-activated protein phosphatase calcineurin functions as a key mediator of diverse biologic processes, including differentiation, apoptosis, growth, and adaptive responses, in part through dephosphorylation and activation of nuclear factor of activated T-cell (NFAT) transcription factors. Apoptosis signal-regulating kinase 1 (ASK1) is an upstream component of the mitogen-activated protein kinases that serves as a pivotal regulator of cytokine-, oxidative-, and stress-induced cell death. Here, we performed a yeast two-hybrid screen with calcineurin B as bait, which identified ASK1 as a direct physical interacting partner. The C-terminal 218 amino acids of ASK1 were sufficient to mediate interaction with calcineurin B in yeast, as well as in mammalian cell lysates. Importantly, endogenous calcium binding B subunit (CnB) protein interacted with endogenous ASK1 protein in cardiomyocytes at baseline, suggesting that the interaction observed in yeast was of potential biologic relevance. Indeed, calcineurin directly dephosphorylated ASK1 at serine 967 using purified proteins or mammalian cell lysates. Dephosphorylation of ASK1 serine 967 by calcineurin promoted its disassociation from 14-3-3 proteins, resulting in...Continue Reading

References

Nov 1, 1995·The Journal of Cell Biology·F Shibasaki, F McKeon
Nov 21, 1997·Biochemical and Biophysical Research Communications·K TobiumeH Ichijo
Apr 21, 1999·Current Opinion in Cell Biology·T P Garrington, G L Johnson
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·L ZhangH Fu
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·J LotemL Sachs
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·T TaigenJ D Molkentin
Aug 10, 2000·The Journal of Biological Chemistry·S SaitoI Komuro
Jan 12, 2001·Molecular and Cellular Biology·A H KimM V Chao
Jun 21, 2001·Molecular and Cellular Biology·X ZouH Kiyokawa
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·J ChenH Fu
May 2, 2002·Cell·Gerald R Crabtree, Eric N Olson
May 17, 2002·The Journal of Physiology·Benjamin J Wilkins, Jeffery D Molkentin
Jan 3, 2003·Nature Cell Biology·Michiko Shirane, Keiichi I Nakayama
Mar 26, 2003·Cell Structure and Function·Kohsuke TakedaHidenori Ichijo
May 17, 2003·The Journal of Clinical Investigation·Julian C BrazJeffery D Molkentin
Sep 17, 2003·Genes & Development·Patrick G HoganAnjana Rao

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Citations

Apr 9, 2011·Cell Death and Differentiation·M NeubertM Schwaninger
Jan 13, 2009·Nature Cell Biology·Qinghang LiuJeffery D Molkentin
Jul 20, 2010·Nature Medicine·Joerg HeinekeJeffery D Molkentin
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kyoung Wan YoonEui-Ju Choi
Jul 21, 2011·The Journal of Biological Chemistry·Hyun-A SeongHyunjung Ha
Jan 25, 2011·The Journal of Biological Chemistry·Marius P Sumandea, Susan F Steinberg
Oct 26, 2010·Antioxidants & Redox Signaling·Kohsuke TakedaHidenori Ichijo
Nov 6, 2010·Antioxidants & Redox Signaling·Christopher RunchelHidenori Ichijo
Jun 3, 2011·Cardiovascular Research·Frank EchtermeyerGregor Theilmeier
Sep 28, 2012·Cardiovascular Research·Jelena KlawitterTamas Seres
May 20, 2009·Molecular and Cellular Biology·Jibin ZhouThomas Force
Sep 22, 2007·Annual Review of Pharmacology and Toxicology·Kohsuke TakedaHidenori Ichijo
Feb 9, 2012·Physiology·Haipeng Sun, Yibin Wang
Jul 18, 2009·Circulation Research·Scott M MacDonnellSteven R Houser
Oct 10, 2009·Circulation Research·Qinghang LiuJeffery D Molkentin
Mar 10, 2012·Circulation Research·Qinghang LiuJeffery D Molkentin
Jul 1, 2007·Future Cardiology·Larissa LipskaiaAnne-Marie Lompre
Jan 19, 2010·Expert Opinion on Biological Therapy·Larissa LipskaiaRoger J Hajjar
Sep 14, 2011·Biochemical and Biophysical Research Communications·Jinbo ChengQun Wei
Mar 29, 2011·Journal of Molecular and Cellular Cardiology·Perundurai S DhandapanyBruce D Gelb
Aug 3, 2010·The International Journal of Biochemistry & Cell Biology·Mikhail A KutuzovAlexandra V Andreeva
Mar 18, 2008·Biochemical Pharmacology·Rajarajan A ThandavarayanYoshifusa Aizawa
Nov 9, 2011·Journal of Molecular and Cellular Cardiology·Joerg Heineke, Oliver Ritter
Mar 26, 2014·Expert Opinion on Therapeutic Targets·Yosuke KawarazakiIsao Naguro
May 31, 2011·Journal of the American College of Cardiology·Dao-Fu DaiPeter S Rabinovitch
Feb 28, 2015·Nature Cell Biology·Diego L MedinaAndrea Ballabio
Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Chihiro SakauchiKazuki Hattori
Feb 27, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Si-Jia LiangJia-Guo Zhou
Nov 30, 2018·The Journal of Physiology·Moriah GildartKimberly L Dodge-Kafka
Jun 11, 2008·The Journal of Biological Chemistry·Michelle A SamaChristopher M Norris
May 1, 2010·Expert Review of Clinical Pharmacology·Ryuji InoueYuko Imai

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