Direct interaction of cellular hnRNP-F and NS1 of influenza A virus accelerates viral replication by modulation of viral transcriptional activity and host gene expression

Virology
Jun Han LeeYoung Ki Choi

Abstract

To investigate novel NS1-interacting proteins, we conducted a yeast two-hybrid analysis, followed by co-immunoprecipitation assays. We identified heterogeneous nuclear ribonucleoprotein F (hnRNP-F) as a cellular protein interacting with NS1 during influenza A virus infection. Co-precipitation assays suggest that interaction between hnRNP-F and NS1 is a common and direct event among human or avian influenza viruses. NS1 and hnRNP-F co-localize in the nucleus of host cells, and the RNA-binding domain of NS1 directly interacts with the GY-rich region of hnRNP-F determined by GST pull-down assays with truncated proteins. Importantly, hnRNP-F expression levels in host cells indicate regulatory role on virus replication. hnRNP-F depletion by small interfering RNA (siRNA) shows 10- to 100-fold increases in virus titers corresponding to enhanced viral RNA polymerase activity. Our results delineate novel mechanism of action by which NS1 accelerates influenza virus replication by modulating normal cellular mRNA processes through direct interaction with cellular hnRNP-F protein.

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C G BurdG Dreyfuss
Aug 1, 1982·The Journal of General Virology·T PetriN J Dimmock
Jan 1, 1993·Annual Review of Biochemistry·G DreyfussC G Burd
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·W M MichaelG Dreyfuss
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiR M Krug
Jul 8, 1999·Current Opinion in Cell Biology·A M Krecic, M S Swanson
Jan 5, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T YoshidaT Tamura
Jul 20, 2001·Journal of Virology·J ParagasP Palese
Mar 15, 2006·The Journal of Experimental Medicine·Rachelle SalomonErich Hoffmann
Apr 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ji-Young Min, Robert M Krug
May 18, 2006·Emerging Infectious Diseases·Thaweesak SongsermYong Poovorawan
Sep 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G HaleRichard E Randall
Feb 8, 2007·Emerging Infectious Diseases·Thaweesak SongsermYong Poovorawan
Apr 20, 2007·Journal of Virology·Georg KochsLuis Martínez-Sobrido
Sep 5, 2007·Biochemical and Biophysical Research Communications·Rikinori MurayamaTorahiko Tanaka
Dec 13, 2007·Biotechnology Journal·Joshua S YuanC Neal Stewart
May 13, 2008·Biochemical and Biophysical Research Communications·Sansanee NoisakranPa-thai Yenchitsomanus
Jul 1, 2008·Virology·Benjamin G HaleRupert J Russell
Jul 23, 2008·Molecular and Cellular Biology·Julian P VenablesBenoit Chabot
Sep 18, 2008·The Journal of General Virology·Benjamin G HaleDavid Jackson
Dec 9, 2008·Biochemical and Biophysical Research Communications·Byounghoon HwangSeong-Wook Lee

❮ Previous
Next ❯

Citations

Dec 19, 2013·PloS One·Bernadette Crescenzo-ChaigneSylvie van der Werf
Jul 24, 2014·Cellular Microbiology·Eike R HrinciusChristina Ehrhardt

❮ Previous
Next ❯

Related Concepts

Related Papers

The Journal of General Virology
Benjamin G HaleDavid Jackson
Biochemical and Biophysical Research Communications
Mami MatsudaMasayuki Noguchi
Proceedings of the National Academy of Sciences of the United States of America
Jasmine T PerezBenjamin R tenOever
© 2022 Meta ULC. All rights reserved