Direct interaction of Sox10 with the promoter of murine Dopachrome Tautomerase (Dct) and synergistic activation of Dct expression with Mitf

Pigment Cell Research
Zhongxian JiaoT J Hornyak

Abstract

The murine dopachrome tautomerase (Dct) gene is expressed early in melanocyte development during embryogenesis, prior to other members of the tyrosinase gene family important for regulating pigmentation. We have used deletion mutants of the Dct promoter, transfections with developmentally relevant transcription factors, and gel shift assays to define transcriptional determinants of Dct expression. Deletion mutagenesis studies show that sequences within the proximal 459 nucleotides are critical for high level expression in melanocytic cells. This region of the promoter contains candidate binding sites for the transcription factors Sox10 and Mitf. Transfections into 293T and NIH3T3 cells show that Sox10 and Mitf independently activate Dct expression, and, when co-transfected, synergistically activate Dct expression. To support the notion that Sox10 acts directly upon the Dct promoter to activate gene expression, direct interaction of Sox10 was demonstrated using gel shifts of oligonucleotide probes derived from promoter sequences within the region required for Sox10-dependent induction. These results suggest that a combinatorial transcription factor interaction is important for expression of Dct in neural crest-derived melanocyte...Continue Reading

References

Apr 25, 1994·Nucleic Acids Research·V R HarleyP N Goodfellow
Feb 3, 1997·The Journal of Experimental Medicine·M B BloomJ C Yang
Jan 13, 1998·Nature Genetics·E M Southard-SmithW J Pavan
Feb 26, 1999·Nucleic Acids Research·M Wegner
Apr 11, 2000·Molecular and Cellular Biology·R I PeiranoM Wegner
Aug 15, 2000·Human Molecular Genetics·N BondurandM Goossens
Mar 7, 2001·Mechanisms of Development·T J HornyakE B Ziff
May 25, 2002·Developmental Biology·Hamid Bolouri, Eric H Davidson
May 29, 2002·The EMBO Journal·Ken-ichi YasumotoShigeki Shibahara
Apr 2, 2003·Human Molecular Genetics·Deborah Lang, Jonathan A Epstein
Jun 26, 2003·Nucleic Acids Research·Scott SchwartzWebb Miller

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Citations

Nov 1, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cheng-Che E LanHsin-Su Yu
Aug 16, 2008·Nucleic Acids Research·C Claus StoltMichael Wegner
Jan 8, 2013·The American Journal of Dermatopathology·Beth PallaSarah Dry
Apr 23, 2008·Pigment Cell & Melanoma Research·Fabien MurisierFriedrich Beermann
Nov 6, 2008·Pigment Cell & Melanoma Research·Jennifer D KubicDeborah Lang
Nov 6, 2008·Pigment Cell & Melanoma Research·Stacie K LoftusWilliam J Pavan
Feb 5, 2009·Pigment Cell & Melanoma Research·Thomas J HornyakFaith M Strickland
May 9, 2009·Pigment Cell & Melanoma Research·Julia C CroninYardena Samuels
Jun 6, 2009·Pigment Cell & Melanoma Research·Stacie K LoftusWilliam J Pavan
Jan 14, 2010·Pigment Cell & Melanoma Research·Joseph B MascarenhasDeborah Lang
May 7, 2010·Pigment Cell & Melanoma Research·Melissa L HarrisWilliam J Pavan
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Thierry PasseronVincent J Hearing
May 17, 2011·Expert Review of Dermatology·Taisuke Kondo, Vincent J Hearing
Aug 29, 2012·The Journal of Cell Biology·Pei-Chih LeeCarole LaBonne
Apr 30, 2009·Critical Reviews in Biochemistry and Molecular Biology·Sandra Medic, Melanie Ziman
Mar 14, 2009·Differentiation; Research in Biological Diversity·Abdelhak BelmadaniRichard J Miller
Dec 16, 2005·The Journal of Investigative Dermatology·Andreya SharovVladimir A Botchkarev
Oct 22, 2008·Seminars in Cell & Developmental Biology·Cynthia D Cooper, David W Raible
May 17, 2005·Pigment Cell Research·Karen Joyce DunnWilliam J Pavan
Jul 21, 2005·Pigment Cell Research·Hideki Murakami, Heinz Arnheiter
Mar 12, 2005·Pigment Cell Research·Michael Wegner
Jul 20, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mayuko KumasakaHiroaki Yamamoto
May 23, 2007·Pigment Cell Research·Fabien MurisierFriedrich Beermann
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Robert N Kelsh
Mar 6, 2010·Experimental Dermatology·Jiri Vachtenheim, Jan Borovanský
Feb 4, 2010·Human Mutation·Véronique PingaultNadège Bondurand
Aug 4, 2005·Seminars in Cell & Developmental Biology·David W Raible, Jared W Ragland
Jul 12, 2005·Peptides·Francois Rouzaud, Vincent J Hearing
Dec 6, 2014·Pigment Cell & Melanoma Research·Jennifer M Huang, Thomas J Hornyak
Jan 18, 2006·Mechanisms of Development·Ramin Mollaaghababa HakamiWilliam J Pavan
Jun 15, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Srinivas Vinod SaladiIvana L de la Serna
Jul 22, 2006·Developmental Biology·Zhongxian JiaoMichael Chopp
Aug 29, 2006·Developmental Biology·Fabien MurisierFriedrich Beermann
May 3, 2006·Developmental Biology·Laure StanchinaNadege Bondurand
Jun 27, 2017·Pigment Cell & Melanoma Research·Hannah E SebergRobert A Cornell
Mar 10, 2016·Development·Oraly Sanchez-FerrasNicolas Pilon
Apr 28, 2009·Molecular BioSystems·Matthew L TomlinsonGrant N Wheeler
Mar 7, 2019·Pigment Cell & Melanoma Research·Eric T DomyanJonathon T Hill

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