Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density.

Neuroscience Letters
Elena O GrachevaJanet Richmond

Abstract

Rim is a multi-domain, active zone protein that regulates exocytosis and is implicated in vesicle priming and presynaptic plasticity. We recently demonstrated that synaptic defects associated with loss of Caenorhabditis elegans Rim (termed UNC-10) are accompanied by a reduction in docked vesicles adjacent to the presynaptic density. Since Rim is known to interact with the vesicle-associated GTPase Rab3A, here we asked whether UNC-10-dependent recruitment of synaptic vesicles to the presynaptic density was through an UNC-10/Rab-3 interaction. We first established that C. elegans Rab3 (termed RAB-3) in its GTP but not GDP-bound state interacts with UNC-10. We then demonstrated by EM analysis that rab-3 mutant synapses exhibit the same vesicle-targeting defect as unc-10 mutants. Furthermore, unc-10;rab-3 double mutants phenocopy the targeting defects of the single mutants, suggesting UNC-10 and RAB-3 act in the same pathway to target vesicles at the presynaptic density. Endogenous release of unc-10;rab-3 double mutants was similar to that of unc-10 single mutants, but more severe than rab-3 mutants, suggesting the common targeting defects are reflected by the milder rab-3 release defect. Rim has recently been shown to positively r...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G Fischer von MollardT C Südhof
Jun 9, 1994·Nature·M GeppertT C Südhof
Jul 5, 2001·The Journal of Biological Chemistry·T CoppolaR Regazzi
Sep 18, 2001·Nature Neuroscience·S P KoushikaM L Nonet
Jan 18, 2002·Nature·Pablo E CastilloRobert C Malenka
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oliver M SchlüterThomas C Südhof
Jul 30, 2005·The EMBO Journal·Irina DulubovaJosep Rizo
Jan 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oliver M SchlüterChristian Rosenmund
Mar 31, 2006·Molecular Biology of the Cell·Timothy R MahoneyMichael L Nonet
May 11, 2006·Journal of Cell Science·Takashi Tsuboi, Mitsunori Fukuda
May 18, 2006·The Journal of Biological Chemistry·Yaisa S Andrews-ZwillingNils Brose
Aug 4, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Robby M WeimerJean-Louis Bessereau
Aug 10, 2006·PLoS Biology·Elena O GrachevaJanet E Richmond

❮ Previous
Next ❯

Citations

Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kaiyun ChenJanet E Richmond
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Pascal S KaeserThomas C Südhof
Dec 2, 2008·PLoS Genetics·Queelim Ch'ngJoshua M Kaplan
Apr 21, 2010·PloS One·Gayla HadwigerMichael L Nonet
Dec 1, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph J BrucknerKate M O'Connor-Giles
Mar 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christian StigloherJean-Louis Bessereau
Jan 7, 2014·Trends in Biochemical Sciences·Daphne Atlas
Nov 28, 2013·Annual Review of Physiology·Pascal S Kaeser, Wade G Regehr
Mar 8, 2012·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Yu-Lin Chiang
Nov 21, 2012·Trends in Neurosciences·Stefan Hallermann, R Angus Silver
Mar 27, 2010·Molecular and Cellular Neurosciences·William L Coleman, Maria Bykhovskaia
Apr 8, 2009·Molecular and Cellular Neurosciences·William L Coleman, Maria Bykhovskaia
Jul 17, 2012·Neuron·Thomas C Südhof
Jun 30, 2009·The European Journal of Neuroscience·Eckart D Gundelfinger, Anna Fejtová
Aug 18, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Minoru TadaToshiaki Katada
Aug 19, 2010·Traffic·Jean-Sébastien SchonnJakob B Sørensen
Jan 27, 2015·Traffic·Devon C Crawford, Ege T Kavalali
Jun 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sangyong JungTobias Moser
Jan 21, 2011·Current Opinion in Neurobiology·Léa SiksouSerge Marty
Apr 24, 2012·Cell Calcium·Jeff W BarclayRobert D Burgoyne
Nov 10, 2017·Frontiers in Synaptic Neuroscience·Tanvi ButolaTobias Moser
Oct 24, 2014·Angewandte Chemie·Thomas C Südhof
May 9, 2019·International Journal of Molecular Sciences·Sumiko Mochida
May 1, 2019·Cellular and Molecular Life Sciences : CMLS·Ardalan HendiKota Mizumoto
Jun 26, 2015·The EMBO Journal·Jerome Di Giovanni, Zu-Hang Sheng
Jun 28, 2015·Biologie aujourd'hui·Thomas C Südhof
Mar 14, 2018·Invertebrate Neuroscience : in·Fernando Calahorro, Patricia G Izquierdo
May 11, 2017·Nature Communications·Marcial CamachoChristian Rosenmund

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.