Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46

The Journal of Biological Chemistry
Ben F LuckerD G Cole

Abstract

Intraflagellar transport (IFT) particles of Chlamydomonas reinhardtii contain two distinct protein complexes, A and B, composed of at least 6 and 15 protein subunits, respectively. As isolated from C. reinhardtii flagella, IFT complex B can be further reduced to a approximately 500-kDa core that contains IFT88, 2x IFT81, 2x IFT74/72, IFT52, IFT46, IFT27, IFT25, and IFT22. In this study, yeast-based two-hybrid analysis was combined with bacterial coexpression to show that three of the core B subunits, IFT88, IFT52, and IFT46, interact directly with each other and, together, are capable of forming a ternary complex. Chemical cross-linking results support the IFT52-IFT88 interaction and provide additional evidence of an association between IFT27 and IFT81. With previous studies showing that IFT81 and IFT74/72 interact to form a (IFT81)(2)(IFT74/72)(2) heterotetramer and that IFT27 and IFT25 form a heterodimer, the architecture of complex B is revealing itself. Last, electroporation of recombinant IFT46 was used to rescue flagellar assembly of a newly identified ift46 mutant and to monitor in vivo localization and movement of the IFT particles.

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L Kindle
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D S Gorman, R P Levine
Jul 1, 1994·The Journal of Cell Biology·Z WaltherJ L Hall
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G KozminskiJoel L Rosenbaum
Jan 1, 1995·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·D N Wheatley
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·G Piperno, K Mead
Feb 9, 1999·Molecular Biology of the Cell·D SignorJonathan M Scholey
Feb 11, 1999·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Mar 9, 1999·Molecular Biology of the Cell·M E PorterW Dentler
Feb 26, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Nov 23, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Jan 3, 2001·Methods : a Companion to Methods in Enzymology·Gregory J Pazour, George B Witman
Mar 17, 2001·Molecular Biology of the Cell·P D TaulmanBradley K Yoder
Feb 8, 2002·American Journal of Physiology. Renal Physiology·Bradley K YoderDaniel F Balkovetz
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Joel L Rosenbaum, George B Witman
Jan 9, 2003·Current Opinion in Cell Biology·Gregory J Pazour, George B Witman
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Jonathan M Scholey
Nov 7, 2003·Nature·Danwei HuangfuKathryn V Anderson
Oct 21, 2004·The Journal of Pathology·B A Afzelius
Feb 16, 2005·Annual Review of Physiology·Helle A Praetorius, Kenneth R Spring
Feb 22, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Junmin PanWilliam J Snell
May 26, 2006·Annual Review of Genomics and Human Genetics·Jose L BadanoNicholas Katsanis
Jul 11, 2006·Developmental Cell·Erica E DavisNicholas Katsanis
Oct 6, 2006·Development·Brent W Bisgrove, H Joseph Yost
Feb 6, 2007·Current Biology : CB·Hongmin QinJoel L Rosenbaum
May 18, 2007·Annual Review of Cell and Developmental Biology·Jonathan T Eggenschwiler, Kathryn V Anderson
Oct 24, 2007·Nature Reviews. Molecular Cell Biology·Manfred FliegaufHeymut Omran
Jan 9, 2008·The Journal of Cell Biology·Wallace F Marshall

Citations

Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Teunis J P van DamMartijn A Huynen
Dec 4, 2012·Bone·An M Nguyen, Christopher R Jacobs
Nov 29, 2011·Differentiation; Research in Biological Diversity·Michael TaschnerEsben Lorentzen
Dec 3, 2014·Developmental Cell·Thibaut EguetherGregory J Pazour
Apr 21, 2011·The EMBO Journal·Sagar BhogarajuEsben Lorentzen
Jun 28, 2013·Cytoskeleton·Caterina MencarelliPietro Lupetti
Aug 16, 2015·Journal of Medical Genetics·Isabelle PerraultFriedhelm Hildebrandt
Jun 12, 2012·Journal of Molecular Biology·Naoko MizunoEsben Lorentzen
Jul 3, 2013·Experimental Cell Research·Paul W HowardRichard A Maurer
Jun 9, 2015·Current Biology : CB·Jason M BrownGeorge B Witman
Feb 28, 2015·Developmental Biology·Mi-Sun LeeCheol-Hee Kim
Jun 21, 2016·Biochemical and Biophysical Research Communications·Inji ParkHyun-Shik Lee
Oct 27, 2017·Small GTPases·Oliver E BlacqueStefanie Kuhns
Jul 14, 2017·Molecular Biology of the Cell·Yuqing Hou, George B Witman
Mar 18, 2017·Journal of Cell Science·Bo LvKaiyao Huang
Mar 28, 2017·The FEBS Journal·Bram PrevoErwin J G Peterman
Mar 10, 2020·Hypertension·Deng-Fu GuoKamal Rahmouni
May 28, 2020·Critical Reviews in Biochemistry and Molecular Biology·Kazuhisa Nakayama, Yohei Katoh
Apr 19, 2017·Cellular and Molecular Life Sciences : CMLS·Xin ZhuJunmin Pan
Jun 30, 2016·Cold Spring Harbor Perspectives in Biology·Michael Taschner, Esben Lorentzen
Jan 19, 2020·Proceedings of the National Academy of Sciences of the United States of America·Bin XueZhen-Chuan Fan
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Benjamin VitreBenedicte Delaval

Related Concepts

IFT88 protein, Chlamydomonas
Carrier Proteins
Cross-Linking Reagents
Flagella
Plant Proteins
Episomes
Plasma Protein Binding Capacity
Proteins, Recombinant DNA
Protozoan Proteins
Mutagenesis Process

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.