Direct isolation of labeled low density lipoproteins for the determination of cholesteryl ester transfer protein activity

Clinica Chimica Acta; International Journal of Clinical Chemistry
D SichI Beucler

Abstract

The measurement of the activity of cholesteryl ester transfer protein (CETP), is of high clinical interest and this study reports the use of a direct LDL isolation (d-LDL) technique to determine in one step the amount of radiolabeled cholesteryls esters ([3H]-CE) transferred from exogenous HDL3 to LDL, avoiding the conveniences of the usually used ultracentrifugation or precipitation of apo-B containing lipoproteins in the CETP methodologies. The d-LDL technique providing a specific immunoprecipitation of VLDL, IDL and HDL allowed to directly determine the [3H]-CE transferred on LDL (d-[3H]-CE-LDL). Two methodologies were assayed for the CETP activity using either exogenous or endogenous lipoproteins, and the results with the d-LDL technique were compared with those obtained using the ultracentrifugation (u-[3H]-CE-LDL) considered as the reference method. The intra- and inter-assays were similar in both techniques for the two CETP activity assays. Strong positive correlations were established between values obtained with d-[3H]-CE-LDL and u-[3H]-CE-LDL isolation procedures for CETP activities with exogenous or endogenous lipoproteins (r = 0.972; p = 0.0001 and r = 0.965; p = 0.0001 respectively). In conclusion, the d-LDL techni...Continue Reading

References

Dec 1, 1991·The Journal of Clinical Investigation·C J MannB E Bihain
Jan 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J J AlbersA Steinmetz
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M GuérinM J Chapman
Jun 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·L LagrostP Gambert
Jun 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·C A MarzettaJ J Albers

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