Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

Journal of Bioscience and Bioengineering
Yuki SomaTaizo Hanai

Abstract

Efficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered Escherichia coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. First, we investigated the cellobiose consumption of two E. coli strains with the BGL protein from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc (Tfu0937/Blc) using different fusion sites. Next, we introduced the synthetic pathway for isopropanol production into those strains and compared their isopropanol production in the presence of glucose. Based on the results of these assays, TA212/pTA411, which was introduced Tfu-Blc fused protein expression system and the synthetic pathway for isopropanol production, was selected for the directly isopropanol production from cellobiose. TA212/pTA411 produced 69.0±11.6mM isopropanol at 21h of fermentation, whereas TA212/pTA147, which did not introduced the BGL/anchor fused protein but was introduced the synthetic pathway for isopropanol production, showed no cellobiose consumption and no isopropanol production during fermen...Continue Reading

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Citations

Mar 13, 2013·Journal of Industrial Microbiology & Biotechnology·Iván Muñoz-Gutiérrez, Alfredo Martinez
Jan 17, 2014·Systems and Synthetic Biology·Thomas LandrainRemi Sussan
Jan 17, 2014·Applied Microbiology and Biotechnology·Shuchi H DesaiShota Atsumi
Aug 12, 2014·Applied Microbiology and Biotechnology·Jan SchüürmannJoachim Jose
Apr 30, 2013·Current Opinion in Chemical Biology·Luisa S GronenbergJames C Liao
Nov 29, 2012·Bioresource Technology·Ethan I Lan, James C Liao
Jun 14, 2015·Biotechnology Advances·Tsutomu Tanaka, Akihiko Kondo
Jan 17, 2014·Bioengineered·Grace L HuangRobert T Clubb
May 6, 2016·Biotechnology Letters·Xiaoyan YangXueming Zhao
May 6, 2015·Chemical Society Reviews·Xinxiao SunQipeng Yuan
Jan 24, 2021·Molecular Biotechnology·Alberto Moura Mendes LopesRosana Goldbeck
Mar 16, 2013·Chemical Reviews·Christine A Rabinovitch-DeereShota Atsumi

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