Direct label-free electrochemical detection of proteins using the polarized oil/water interface

Langmuir : the ACS Journal of Surfaces and Colloids
Toshiyuki OsakaiHajime Katano

Abstract

Voltammetric behaviors of various globular proteins, including cytochrome c, ribonuclease A, lysozyme, albumin, myoglobin, and alpha-lactalbumin, were studied at the polarized 1,2-dichloroethane/water (DCE/W) interface in the presence of four different anionic surfactants, that is, dinonylnaphthalenesulfonate (DNNS), bis(2-ethylhexyl)sulfosuccinate (Aerosol-OT; AOT), bis(2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptyl)sulfosuccinate (BDFHS), and bis(2-ethylhexyl)phosphate (BEHP). When the W phase was acidic (pH = approximately 3.4), the surfactants (except for BEHP) added to DCE facilitated the adsorption of the above proteins to the DCE/W interface and gave a well-developed voltammetric wave due to the adsorption/desorption of the proteins. This voltammetric wave, which we here call "protein wave", is promising for direct label-free electrochemical detection of proteins. The current for the adsorption of a protein to the interface showed a linear dependence on the protein concentration in the presence of excess surfactant. The foot potential at which the protein wave appeared in cyclic voltammetry showed different values depending on the natures of the protein and surfactant. Multivariate analysis for the foot potentials determined...Continue Reading

Citations

Dec 15, 2012·Analytical and Bioanalytical Chemistry·Eva Alvarez de EulateDamien W M Arrigan
Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Dec 25, 2012·Analytical Chemistry·Shane O'Sullivan, Damien W M Arrigan
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan
Dec 18, 2020·Bioelectrochemistry·Shaheda Zannah, Damien W M Arrigan

Related Concepts

Ethylene dichloride, ion (1+)
1,4-bis(2-ethylhexyl) sodium sulfosuccinate
Electrochemistry
Ethylene Dichlorides
Leftose
Myoglobin
Oils
Succinic Acids
Tensides
Adsorption

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