Direct link from the suprachiasmatic nucleus to hypothalamic neurons projecting to the spinal cord: a combined tracing study using cholera toxin subunit B and Phaseolus vulgaris-leucoagglutinin

Brain Research Bulletin
N VrangP J Larsen

Abstract

By combining retrograde and anterograde tracing, evidence for a bineuronal connection from the suprachiasmatic nucleus (SCN) to the intermediolateral cell column in the spinal cord (IML) was obtained. The retrograde tracer cholera toxin subunit B (ChB) was pressure-injected into the spinal cord and the anterograde tracer Phaseolus vulgaris-leucoagglutinin (PHA-L) was iontophoretically injected into the SCN. The two tracers were visualized simultaneously by a double immunohistochemical procedure. In the hypothalamus, ChB injections gave rise to retrogradely labeled cell bodies in the paraventricular nucleus, retrochiasmatic area, perifornical region, lateral hypothalamic area, and the posterior hypothalamic area. The SCN were found to project to all of these areas. Furthermore, spinal-projecting neurons were found in the brain stem, but no efferents from the SCN were observed to innervate these areas. In the most sparsely innervated areas, the lateral hypothalamic area and the perifornical region, only occasionally a PHA-L fiber in close apposition to a ChB-ir cell body was observed. This was also the case in the retrochiasmatic area and posterior hypothalamic area, although these areas received a moderate number-immunoreactive ...Continue Reading

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Citations

Jul 2, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carrie E MahoneyEric L Bittman
Jun 3, 2011·The Journal of Clinical Investigation·Wenyu HuangJoseph Bass
Feb 27, 2004·The Journal of Comparative Neurology·Chiara FabrisMorten Møller
Mar 19, 2011·FEBS Letters·Andries KalsbeekRuud M Buijs
Aug 6, 2013·BioFactors·Charlene DiepenbroekSusanne E la Fleur
Sep 20, 2006·Progress in Neurobiology·Norman PecoraroSusan F Akana
Aug 6, 1999·Frontiers in Neuroendocrinology·Y IbataF Amaya
Mar 30, 1999·The Journal of Comparative Neurology·R Teclemariam-MesbahR M Buijs
Jan 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Dominique SageOlivier Bosler
Feb 26, 2019·Industrial Health·Alfredo Copertaro, Massimo Bracci
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·F A ScheerR M Buijs
Sep 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L N CuiL P Renaud

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