PMID: 9174284Apr 11, 1997Paper

Direct liquid chromatographic micro-measurement of tamoxifen in plasma of cancer patients

Journal of Chromatography. B, Biomedical Sciences and Applications
A el-Yazigi, E Legayada

Abstract

We describe in this report a sensitive and direct method for the analysis of tamoxifen (TAM) in microsamples of plasma. The drug and internal standard (quinine bisulfate, I.S.) were separated on a 10-microm particle, 10 cm X 8 mm CN cartridge in conjunction with a radial compression system. The mobile phase was a mixture of 0.1 M sodium acetate in 0.001 M tetrabutylammonium phosphate solution (pH 6) and methanol (30:70, v/v) at a flow-rate of 4 ml/min. After addition of I.S. and o-phosphoric acid in acetonitrile (0.6 M) to the plasma (30 microl), the mixture was placed in an ultraviolet shortwave transluminator for 2 min prior to injection into the chromatograph. The compounds were detected in the effluent fluorometrically at excitation and emission wavelengths of 258 and 378 nm, respectively. Under these conditions, no interference in the assay from any endogenous substance or other concurrently used drugs was observed and the retention times of I.S. and TAM were 4.4 and 10.15 min, respectively. The concentration of TAM in plasma was linearly (r>0.9983) related to the peak height ratio (TAM/I.S.) in the range 0.01-2.0 microg ml(-1) and C.V. at 0.075, 0.4 and 1.2 microg ml(-1) was < or = 4.96%. We are currently using this assay...Continue Reading

References

Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J MillwardA L Harris
May 13, 1994·Journal of Chromatography. B, Biomedical Applications·K M Fried, I W Wainer
Nov 1, 1993·Biomedical Chromatography : BMC·C K LimL L Smith

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Citations

Nov 26, 2010·Analytica Chimica Acta·S F TeunissenJ H Beijnen
Jan 8, 2013·Journal of Pharmaceutical and Biomedical Analysis·Marina Venzon AntunesRafael Linden

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