Direct measurement of dipole-dipole/CSA cross-correlated relaxation by a constant-time experiment.

Journal of Magnetic Resonance
Yizhou Liu, J H Prestegard

Abstract

Relaxation rates in NMR are usually measured by intensity modulation as a function of a relaxation delay during which the relaxation mechanism of interest is effective. Other mechanisms are often suppressed during the relaxation delay by pulse sequences which eliminate their effects, or cancel their effects when two data sets with appropriate combinations of relaxation rate effects are added. Cross-correlated relaxation (CCR) involving dipole-dipole and CSA interactions differ from auto-correlated relaxation (ACR) in that the signs of contributions can be changed by inverting the state of one spin involved in the dipole-dipole interaction. This property has been exploited previously using CPMG sequences to refocus CCR while ACR evolves. Here we report a new pulse scheme that instead eliminates intensity modulation by ACR and thus allows direct measurement of CCR. The sequence uses a constant time relaxation period for which the contribution of ACR does not change. An inversion pulse is applied at various points in the sequence to effect a decay that depends on CCR only. A 2-D experiment is also described in which chemical shift evolution in the indirect dimension can share the same constant period. This improves sensitivity by ...Continue Reading

References

Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R RiekK Wüthrich
Apr 26, 2003·Journal of Magnetic Resonance·Philippe PelupessyGeoffrey Bodenhausen
Mar 18, 2005·Journal of Biomolecular NMR·Paul R VasosDavid Fushman
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Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Crislyn D'Souza-Schorey, Philippe Chavrier

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Citations

Jul 6, 2010·Nature Structural & Molecular Biology·Yizhou LiuJames H Prestegard
Nov 22, 2014·Nature Communications·Rama K KoppisettiSteven R Van Doren
Jan 19, 2010·Journal of Molecular Biology·Caroline M Maynard, Kathleen B Hall
Dec 15, 2010·Protein Science : a Publication of the Protein Society·Adam W BarbJames H Prestegard
Jul 27, 2010·Biophysical Journal·Xiangyang LiangSteven R Van Doren
Jan 15, 2009·Structure·Yizhou LiuJames H Prestegard
Jul 8, 2009·Journal of Magnetic Resonance·Yizhou Liu, James H Prestegard

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