PMID: 8603085Mar 13, 1996Paper

Direct measurement of ion distributions between lipid membranes with X-ray diffraction

Biochimica Et Biophysica Acta
S Kirchner

Abstract

A new and simple method is introduced, which allows the direct measurement of the distribution of ions between lipid membranes with a conventional X-ray source. It is based on a difference method which is combined with a swelling experiment. The presented method is applied to unoriented powder samples of 1,2-dipalmitoyl-sn-glycero-3-phosphorylglycerol in different ionic solutions of RbCl and BaCl2. From these samples, results for the cation distributions with a resolution of 12 A degrees were obtained. Analysis of the experimentally obtained distributions shows that the simple Gouy-Chapman theory is probably not able to describe the experimental data consistently. Instead a better correspondence between experiment and theory is obtained with a generalized linear Gouy-Chapman model which takes into account the finite width of the lipid/electrolyte interface. Possible future improvements of the presented method with regard to the obtained resolution and the possibility to obtain ion densities on an absolute scale are discussed.

References

Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Jul 15, 1986·Biochemistry·T J McIntosh, S A Simon
Jan 1, 1986·Chemistry and Physics of Lipids·K K Eklund, P K Kinnunen

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