Direct measurement of nitric oxide release in gastric mucosa during ischemia-reperfusion in rats

The American Journal of Physiology
K WadaT Itoh

Abstract

Nitric oxide (NO) generation in the rat gastric mucosa during ischemia-reperfusion was measured using an NO-sensitive electrode. Under pentobarbital sodium anesthesia, an electrode was inserted into the submucosa from the serous membrane side in the fundus. After steady-state baseline recording, the celiac artery was clamped for 30 min, and then ischemia-reperfusion was achieved by removing the clamp. The clamping of the celiac artery caused a decrease in blood flow and an increase in NO level in the gastric tissue. Just after the removal of the clamp, the NO level rapidly fell and returned to the baseline level. Administration of NG-nitro-L-arginine methyl ester (an NO synthase inhibitor, 30 mg/kg i.p.) before ischemia significantly attenuated both the increase in NO level during ischemia and the formation of acute gastric mucosal lesions observed after 60 min reperfusion. Administration of superoxide dismutase (a superoxide radical scavenger, 10,000 U/kg i.v.) at the end of ischemia inhibited both the rapid decrease in NO level during the reperfusion and the gastric mucosal erosions. Because NO and superoxide radical produce a highly reactive peroxynitrite, it can be argued that NO has an important pathological role in acute ...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·R G Knowles, S Moncada
Jan 1, 1992·Trends in Pharmacological Sciences·T McCall, P Vallance
Aug 1, 1991·Archives of Biochemistry and Biophysics·R RadiB A Freeman
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Feb 1, 1986·Gastroenterology·M A PerryD N Granger
Nov 1, 1994·Canadian Journal of Physiology and Pharmacology·B L Tepperman, B D Soper
Nov 1, 1994·Shock·H MitsuhataR Shimizu
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D RothsteinR W Kuncl
Feb 1, 1994·Digestive Diseases and Sciences·F J AndrewsP E O'Brien
May 1, 1993·Biochemical Society Transactions·K J Davies
May 1, 1993·Biochemical Society Transactions·K FairburnD R Blake
Jan 1, 1993·British Journal of Pharmacology·J Lopez-BelmonteS Moncada

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Citations

Jul 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Koichiro WadaRichard S Blumberg
Mar 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael C MontaltoGregory L Stahl
Aug 24, 2000·Journal of Applied Physiology·R IturriagaM Mosqueira
Apr 26, 2008·Journal of Clinical Biochemistry and Nutrition·Makoto Sasaki, Takashi Joh
May 30, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Naohisa KuriyamaShinji Uemoto
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·C TsujiH Nakazawa

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