Direct measurement of oscillatory generation of superoxide anions by single phagocytes

FEBS Letters
Y IsogaiK Tanaka

Abstract

Phagocytic cells such as neutrophils generate superoxide anions (O2-) within phagocytic vacuoles for killing and digesting microorganisms. Here we report the simultaneous observation of morphological changes and O2- generation in single phagocytic cells during phagocytosis. Point stimulation of a cell by contact with an opsonized microelectrode at the cell surface induced significant deformation to engulf the electrode, and also induced the O2- generation which was measured by the electrode. Periodic fluctuations in the magnitude of the O2- generation were observed in the time course. These oscillations may be caused by metabolic regulation of the formation of NADPH, which is the substrate for the O2- generation

References

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Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Jan 15, 1993·Science·A VermaS H Snyder
Apr 7, 1995·The Journal of Biological Chemistry·Y IsogaiY Shiro

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