Direct measurement of oscillatory RhoA activity in embryonic cortical neurons stimulated with the axon guidance cue netrin-1 using fluorescence resonance energy transfer

Biology of the Cell
Judith Antoine-BertrandNathalie Lamarche-Vane

Abstract

Rho GTPases play an essential role during the development of the nervous system. They induce cytoskeletal rearrangements that are critical for the regulation of axon outgrowth and guidance. It is generally accepted that Rac1 and Cdc42 are positive regulators of axon outgrowth and guidance, whereas RhoA is a negative regulator. However, spatiotemporal control of their activity can modify the function of Rho GTPases during axonal morphogenesis. Signalling downstream of the axon guidance cue netrin-1 and its receptor deleted in colorectal cancer (DCC) triggers the activation of Rac1 and the inhibition of RhoA to promote axon outgrowth. However, our previous work also suggests that netrin-1/DCC signalling can activate RhoA in a time- and region-specific manner. Here, we visualised RhoA activation in response to netrin-1 in live embryonic cortical neurons using fluorescence resonance energy transfer. RhoA activity oscillated in unstimulated neurons and netrin-1 increased the amplitude of the oscillations in growth cones after 5 min of stimulation. Within this period of time, netrin-1 transiently increased RhoA activity and modulated the pattern of RhoA oscillations. We found that the timing of netrin-1-induced RhoA activation was di...Continue Reading

References

Jun 10, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M AmanoK Kaibuchi
May 8, 1999·Molecular Biology of the Cell·L Castelo, D G Jay
Feb 28, 2002·The Journal of Biological Chemistry·Songhee JeonJoobae Park
May 30, 2002·The Journal of Cell Biology·Olivier GenesteRichard Treisman
Dec 25, 2002·Journal of Neurobiology·Edda Thies, Roger W Davenport
Apr 23, 2003·The Journal of Cell Biology·Yoshiki ArakawaShuh Narumiya
Oct 1, 2003·The Journal of Cell Biology·Jorge Santos Da SilvaCarlos G Dotti
Mar 3, 2004·The Journal of Cell Biology·Bruno T FievetMonique Arpin
Mar 27, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erik W DentKatherine Kalil
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ling ChengVance Lemmon
Jun 1, 2005·Biophysical Journal·Yuichi SakumuraShin Ishii
Jul 19, 2005·Brain Research. Molecular Brain Research·Takeshi NakamuraMichiyuki Matsuda
Aug 25, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Timothy E KennedyMarc Tessier-Lavigne
Jul 9, 2008·Developmental Cell·Dylan T BurnettePaul Forscher
Sep 12, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Soichiro OhnamiNobuhiko Yamamoto
Apr 18, 2009·Nature Reviews. Molecular Cell Biology·Laura Anne Lowery, David Van Vactor
Apr 30, 2009·Annual Review of Neuroscience·Michael O'DonnellGreg J Bashaw
Aug 21, 2009·Nature·Matthias MachacekGaudenz Danuser
Sep 1, 2009·Trends in Cell Biology·Grégory GiannoneOlivier Thoumine
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Alan Hall, Giovanna Lalli
Mar 24, 2010·Nature Reviews. Molecular Cell Biology·Richard G FehonAnthony Bretscher
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Greg J Bashaw, Rüdiger Klein
May 21, 2010·Journal of Cell Science·Olivier Pertz
May 28, 2010·Developmental Neurobiology·Bonnie M MarsickPaul C Letourneau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.