Direct measurement of pH profiles in immobilized enzyme carriers during kinetically controlled synthesis using CLSM

Biotechnology Progress
A C Spiess, V Kasche

Abstract

Confocal laser scanning microscopy was applied to measure the pH value in the carrier of immobilized enzymes during the enzyme-catalyzed synthesis. pH profiles with a high resolution are shown, with the pH increasing in the core of the particles. Significant differences occur for different carrier material, particle size, porosity and surface modification. The increased pH value is identified as one of the reasons leading to reduced enzyme selectivity in the penicillin amidase catalyzed synthesis of cephalosporins and penicillins.

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Citations

May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Volker KascheMoustafa Gad
Sep 27, 2002·Current Opinion in Biotechnology·Jeroen van RoonHans Tramper
Jan 7, 2006·Applied Microbiology and Biotechnology·J L van RoonH H Beeftink
Feb 7, 2013·Trends in Biotechnology·Juan M BolivarBernd Nidetzky
Aug 5, 2014·Journal of Microencapsulation·Xiao Yan LiMin Zhao
Oct 13, 2012·Chemical Society Reviews·Rafael C RodriguesRoberto Fernández-Lafuente
Jul 13, 2002·Biotechnology and Bioengineering·Luuk M Van LangenRoger A Sheldon
Apr 12, 2002·Biotechnology and Bioengineering·Michiel H A JanssenRoger A Sheldon
Sep 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan M Bolivar, Bernd Nidetzky

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