PMID: 8610155Apr 2, 1996Paper

Direct measurement of salt-bridge solvation energies using a peptide model system: implications for protein stability

Proceedings of the National Academy of Sciences of the United States of America
W C WimleyS H White

Abstract

The solvation energies of salt bridges formed between the terminal carboxyl of the host pentapeptide AcWL- X-LL and the side chains of Arg or Lys in the guest (X) position have been measured. The energies were derived from octanol-to-buffer transfer free energies determined between pH 1 and pH 9. 13C NMR measurements show that the salt bridges form in the octanol phase, but not in the buffer phase, when the side chains and the terminal carboxyl group are charged. The free energy of salt-bridge formation in octanol is approximately -4 kcal/mol (1 cal = 4.184 J), which is equal to or slightly larger than the sum of the solvation energies of noninteracting pairs of charged side chains. This is about one-half the free energy that would result from replacing a charge pair in octanol with a pair of hydrophobic residues of moderate size. Therefore, salt bridging in octanol can change the favorable aqueous solvation energy of a pair of oppositely charged residues to neutral or slightly unfavorable but cannot provide the same free energy decrease as hydrophobic residues. This is consistent with recent computational and experimental studies of protein stability.

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·F M Richards
May 5, 1992·Journal of Molecular Biology·L Holm, C Sander
Jul 5, 1992·Journal of Molecular Biology·C N Pace
Jan 16, 1986·Nature·D Eisenberg, A D McLachlan
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B H Honig, W L Hubbell
Aug 25, 1983·Journal of Molecular Biology·D J Barlow, J M Thornton
Apr 21, 1995·Journal of Molecular Biology·O Schueler, H Margalit
Feb 1, 1995·Nature Structural Biology·C D WaldburgerR T Sauer
Feb 1, 1995·Nature Structural Biology·B W Matthews
Feb 1, 1994·Protein Science : a Publication of the Protein Society·Z S Hendsch, B Tidor
Jun 1, 1994·Proteins·T P Creamer, G D Rose
May 1, 1993·Journal of Pharmaceutical Sciences·N P FranksW R Lieb

❮ Previous
Next ❯

Citations

Mar 26, 1998·Protein Science : a Publication of the Protein Society·L T ChongB Tidor
Oct 7, 1998·Protein Science : a Publication of the Protein Society·C V SindelarB Tidor
May 26, 2009·European Biophysics Journal : EBJ·Valery G Veresov, Alexander I Davidovskii
Mar 29, 2003·Journal of Molecular Biology·George I MakhatadzeSusan T Thomas
Jan 12, 2013·Journal of Chemical Information and Modeling·Hajira Ahmed Hotiana, Muhammad Kamran Haider
Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
Aug 4, 2012·The Journal of Biological Chemistry·Christopher C ValleyJonathan N Sachs
Nov 21, 2002·Protein Science : a Publication of the Protein Society·Chien Peter ChenBurkhard Rost
Mar 27, 2001·Protein Science : a Publication of the Protein Society·L P Lee, B Tidor
Jun 13, 2000·Protein Science : a Publication of the Protein Society·H Im, M H Yu
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Jun 25, 2008·Annual Review of Biophysics·Stephen H White, Gunnar von Heijne
Jul 21, 2004·The Journal of Chemical Physics·Edward Z Wen, Ray Luo
Nov 21, 2012·The Journal of Chemical Physics·Eva PluhařováPavel Jungwirth
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R C WadeV Lounnas
May 15, 2012·Annual Review of Biophysics·Arianna Rath, Charles M Deber
Apr 29, 2014·Biochimica Et Biophysica Acta·Paulo F Almeida
Mar 11, 2010·Biochimica Et Biophysica Acta·Mary Rose G Tandang-SilvasNobuyuki Maruyama
Aug 25, 2004·Proteins·Brian N DominyCharles L Brooks
Jun 7, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Petra RovóAndrás Perczel
Jan 5, 2014·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Seth BlumenthalBrian Y Chen
Nov 3, 2004·Biochimica Et Biophysica Acta·Walter L AshD Peter Tieleman
Apr 28, 2005·Journal of Molecular Biology·Jinrang KimM R Gunner
Jun 8, 2014·Current Opinion in Structural Biology·Torsten H Walther, Anne S Ulrich
Nov 18, 2000·Structure·T KajanderA Goldman
Apr 29, 2009·Biochimica Et Biophysica Acta·Barbara OrioniLorenzo Stella

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.