Abstract
Site-directed mutants of the herpes simplex virus type 1 uracil-DNA glycosylase lacking catalytic activity have been used to probe the substrate recognition of this highly conserved and ubiquitous class of DNA-repair enzyme utilizing surface plasmon resonance. The residues aspartic acid-88 and histidine-210, implicated in the catalytic mechanism of the enzyme (Savva, R., McAuley-Hecht, K., Brown, T., and Pearl, L. (1995) Nature 373, 487-493; Slupphaug, G., Mol, C. D., Kavli, B., Arvai, A. S., Krokan, H. E. and Tainer, J. A. (1996) Nature 384, 87-92) were separately mutated to asparagine to allow investigations of substrate recognition in the absence of catalysis. The mutants were shown to be correctly folded and to lack catalytic activity. Binding to single- and double-stranded oligonucleotides, with or without uracil, was monitored by real-time biomolecular interaction analysis using surface plasmon resonance. Both mutants exhibited comparable rates of binding and dissociation on the same uracil-containing substrates. Interaction with single-stranded uracil-DNA was found to be stronger than with double-stranded uracil-DNA, and the binding to Gua:Ura mismatches was significantly stronger than that to Ade:Ura base pairs suggesti...Continue Reading
References
Oct 6, 1975·Biochimica Et Biophysica Acta·K J ChangP Cuatrecasas
Jan 1, 1992·Chromosoma·F FocherS Spadari
Nov 24, 1981·Biochemistry·O G BergP H von Hippel
Jul 1, 1995·Proteins·R Savva, L H Pearl
Sep 1, 1995·Nature Structural Biology·R Savva, L H Pearl
Jun 30, 1995·Science·H W ParkJ Deisenhofer
Jul 14, 1995·Cell·R J Roberts
Sep 8, 1995·Cell·C D MolJ A Tainer
Aug 1, 1993·Mutation Research·M Higley, R S Lloyd
Mar 24, 1995·Cell·C D MolJ A Tainer
Feb 9, 1995·Nature·R SavvaL Pearl
Feb 1, 1994·Protein Science : a Publication of the Protein Society·R J FisherT Papas
Oct 1, 1993·Analytical Biochemistry·K BondesonG Magnusson
Dec 5, 1993·Journal of Molecular Biology·R Savva, L H Pearl
Jan 28, 1994·Cell·S KlimasauskasX Cheng
Apr 22, 1993·Nature·T Lindahl
Dec 1, 1995·Cell·D G VassylyevK Morikawa
Oct 1, 1996·FEMS Microbiology Letters·A KoulisR Savva
Oct 18, 1996·Cell·E SpanopoulouG Panayotou
Nov 7, 1996·Nature·G SlupphaugJ A Tainer
Citations
Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Nov 6, 2002·Journal of Molecular Biology·Monika FuxreiterRoman Osman
Aug 18, 2000·Mutation Research·L H Pearl
Aug 18, 2000·Mutation Research·S S ParikhJ A Tainer
Jun 25, 2003·Molecular Cell·Jane E A WibleyLaurence H Pearl
Nov 9, 2002·European Journal of Biochemistry·Mark J DickmanDavid P Hornby
Feb 24, 2001·Nucleosides, Nucleotides & Nucleic Acids·A RöslerG M Blackburn
Sep 2, 1998·The EMBO Journal·S S ParikhJ A Tainer
Sep 15, 2001·Nucleic Acids Research·X Cheng, R J Roberts
Aug 19, 2007·Nucleic Acids Research·Gemma Serrano-HerasMargarita Salas
Sep 24, 2010·Journal of Virology·Abigail M Druck ShudofskyRobert P Ricciardi
Nov 2, 2001·Journal of Virology·S MisumiS Shoji
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C D MolJ A Tainer
Apr 18, 2014·PloS One·Scott T KimberKeith R Fox
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A GreaggL H Pearl
Oct 3, 2015·Nucleic Acids Research·Gergely RónaBeáta G Vértessy
Sep 26, 2013·Critical Reviews in Microbiology·Marco RusnatiChiara Urbinati
Mar 7, 1998·Cell·T E BarrettL H Pearl
Mar 23, 2018·Physical Chemistry Chemical Physics : PCCP·Neeladri Sekhar RoySiddhartha Roy
Mar 30, 2001·The Journal of Biological Chemistry·V K ShierS J Benkovic
Aug 2, 2017·PloS One·Eunju KwonDong Young Kim
Jul 23, 2008·The Analyst·Christelle CorneAlain Favier
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·A PooleB M Sjöberg
Jan 20, 2006·The Journal of Biological Chemistry·Gemma Serrano-HerasAlicia Bravo
Jun 6, 2015·The Journal of Biological Chemistry·Wim P BurmeisterFrédéric Iseni
Sep 21, 2004·The Journal of Biological Chemistry·Adesh Kumar SainiVibha Tandon
Nov 30, 2019·Scientific Reports·Kathrin HölzMark M Somoza
Jul 13, 2000·The Journal of Biological Chemistry·S KumarL H Pearl
Nov 25, 2005·The Journal of Biological Chemistry·Kuakarun KrusongGeoff S Baldwin
Nov 26, 2009·Journal of the American Chemical Society·Anna Zhachkina, Jeehiun K Lee
Jul 13, 2006·The Journal of Physical Chemistry. a·Patric SchymanAatto Laaksonen
Apr 7, 2004·Biochemistry·Daniel J KroskyJames T Stivers
May 19, 2007·The Journal of Physical Chemistry. B·Matteo CalvaresiMarco Garavelli
Jun 6, 2003·Journal of the American Chemical Society·John H MillerMichael A Kennedy
Sep 11, 2002·Biochemistry·Eleanore SeibertRoman Osman
Dec 22, 1999·Biochemistry·D C Carey, P R Strauss
Feb 9, 2006·Journal of the American Chemical Society·Jianguo Fang, Arne Holmgren
Apr 14, 1999·Biochemistry·M J ShroyerD W Mosbaugh