Direct measurements of convective fluid velocities in superporous agarose beads

Journal of Chromatography. a
P E GustavssonP O Larsson

Abstract

Superporous agarose beads contain two sets of pores, diffusion pores and so-called superpores or flow pores, in which the chromatographic flow can transport substances to the interior of each individual bead [Gustavsson and Larsson, J. Chromatogr. A 734 (1996) 231]. The existence of pore flow may be proven indirectly by the chromatographic performance of beads but it has never been directly demonstrated in a chromatographic bed. In this report, pore flow was directly measured by following the movement of micro-particles (dyed yeast cells) in a packed bed. The passage of the micro-particles through the superpores and through the interstitial pores was followed by a microscope/video camera focused on beads which were situated four layers from the glass wall. The video recordings were subsequently used to determine the convective fluid velocities in both the superpores and the interstitial pores. Experiments were carried out with three different bead size ranges, all of which contained superporous beads having an average superpore diameter of 30 microns. The superpore fluid velocity as % of interstitial fluid velocity was determined to be 2-5% for columns packed with 300-500-micron beads (3% average value), 6-12% for columns packe...Continue Reading

Citations

Apr 10, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ingo GottschalkPer Lundahl
Oct 16, 2007·Journal of Biotechnology·B Balasundaram, S T L Harrison
Sep 10, 2004·Journal of Separation Science·Fatima M PlievaBo Mattiasson
Sep 10, 2004·Journal of Separation Science·Alfred ZöchlingAlois Jungbauer
Nov 2, 2005·Biotechnology and Bioengineering·Qing-Hong ShiYan Sun
Oct 31, 2006·Journal of Chromatography. a·Peter TiainenPer-Olof Larsson
Nov 15, 2018·Journal of Separation Science·Aleš Podgornik
Apr 19, 2019·Micromachines·Nicolaos ChristodoulidesJohn T McDevitt
Feb 27, 2010·Analytical Chemistry·Véronique WernertRenaud Denoyel
Jan 19, 2012·Analytical Chemistry·Jie ChouJohn T McDevitt
Nov 6, 2007·Analytical Chemistry·M BarrandeR Denoyel

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