PMID: 8953700Nov 1, 1996Paper

Direct methods in protein electron crystallography: the ab initio structure determination of two membrane protein structures in projection using maximum entropy and likelihood

Acta Crystallographica. Section A, Foundations of Crystallography
C J GilmoreD L Dorset

Abstract

Using maximum entropy and likelihood, an ab initio phase determination was carried out in projection at ca 6-10 A resolution for two dissimilar membrane proteins: the Omp F porin from the outer membrane of E. coli (largely beta-sheet) and halorhodopsin (largely alpha-helix). Accurate phase information found for the most likely solutions enabled potential maps to be calculated that contained most of the essential structural details of these macromolecules without the need for any image-derived phases as a starting set for phase extension or the necessity to use envelopes or electron-density histograms. A comparison with earlier calculations using the Sayre-Hughes equation coupled with phase annealing and the Luzzati flatness criterion used as a figure of merit is made.

Citations

Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·D L Dorset
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·D L Dorset
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·D L Dorset
Jan 24, 2007·Ultramicroscopy·Douglas L Dorset
Jul 29, 1999·Microscopy Research and Technique·D L Dorset
Jul 29, 1999·Microscopy Research and Technique·C J Gilmore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Micron : the International Research and Review Journal for Microscopy
D L Dorset
Acta Crystallographica. Section D, Biological Crystallography
V Y LuninA D Podjarny
Acta Crystallographica. Section D, Biological Crystallography
T C Terwilliger
© 2021 Meta ULC. All rights reserved