Direct microscopic observation of viability of Campylobacter jejuni on chicken skin treated with selected chemical sanitizing agents

Journal of Food Protection
Walairut ChantarapanontJ F Frank

Abstract

The objective of this research was to determine the effect of chlorine, acidified sodium chlorite, and peracetic acid treatments on viable Campylobacter jejuni located at various depths within follicles or folds of chicken skin. Chicken skin was inoculated with C. jejuni transformed with P(c)gfp plasmid (GFP-Campylobacter), which also codes for kanamycin resistance. Effectiveness of sanitizer treatments was determined by plate count. C. jejuni were also observed on chicken skin by confocal scanning laser microscopy, whereby viable and nonviable cells were differentiated by their ability to take up staining with 5-cyano-2,3-ditolyl tetrazolium chloride. Sodium hypochlorite, peracetic acid, and acidified sodium chlorite were each applied at 40 or 100 ppm for 2 or 15 min. Each sanitizer resulted in approximately a 1-log decrease (CFU) when used at 100 ppm for 15 min and no significant decrease when used at 40 ppm for 2 min. Numbers of viable cells observed on the skin by direct microscopic count were similar to numbers obtained by plate count. Although viable counts decreased with sanitizer treatments, the total number of Campylobacter cells (live plus dead) attached to the skin remained unchanged. After each chemical treatment, v...Continue Reading

References

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Dec 16, 2003·Journal of Food Protection·Walairut ChantarapanontJoseph F Frank
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Citations

Mar 1, 2012·European Journal of Microbiology & Immunology·K StinglA Käsbohrer
May 24, 2006·Journal of Food Protection·Norman J Stern, Stephen Pretanik
Jul 21, 2009·Journal of Food Protection·Charlotte Tandrup RiedelBjarke Bak Christensen
Apr 19, 2006·Avian Diseases·Margie D Lee, Diane G Newell
May 13, 2017·The Journal of Veterinary Medical Science·Chiharu ToyofukuKazuaki Takehara
Jul 11, 2020·Virology Journal·Robert Joseph AtterburyVivien Mary Allen
Nov 7, 2020·International Journal of Food Microbiology·Takako TaniguchiNaoaki Misawa
Mar 17, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Alicia TorralboAlfonso Carbonero

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