Direct modulation of phospholipase D activity by Gbetagamma.

Molecular Pharmacology
Anita M PreiningerH Alex Brown

Abstract

Phospholipase D-mediated hydrolysis of phosphatidylcholine is stimulated by protein kinase C and the monomeric G proteins Arf, RhoA, Cdc42, and Rac1, resulting in complex regulation of this enzyme. Using purified proteins, we have identified a novel inhibitor of phospholipase D activity, Gbetagamma subunits of heterotrimeric G proteins. G protein-coupled receptor activation alters affinity between Galpha and Gbetagamma subunits, allowing subsequent interaction with distinct effectors. Gbeta1gamma1 inhibited phospholipase D1 and phospholipase D2 activity, and both Gbeta1gamma1 and Gbeta1gamma2 inhibited stimulated phospholipase D1 activity in a dosedependent manner in reconstitution assays. Reconstitution assays suggest this interaction occurs through the amino terminus of phospholipase D, because Gbeta1gamma1 is unable to inhibit an amino-terminally truncated phospholipase D construct, PLD1.d311, which like full-length phospholipase D isoforms, requires phosphatidylinositol-4,5-bisphosphate for activity. Furthermore, a truncated protein consisting of the amino-terminal region of phospholipase D containing the phox/pleckstrin homology domains was found to interact with Gbeta1gamma1, unlike the PLD1.d311 recombinant protein, whic...Continue Reading

References

Jun 23, 1995·The Journal of Biological Chemistry·H A BrownP C Sternweis
Jun 23, 1995·The Journal of Biological Chemistry·W D SingerP C Sternweis
May 16, 1997·The Journal of Biological Chemistry·K M EllaK E Meier
Dec 29, 1998·The Journal of Biological Chemistry·T C SungM A Frohman
Jan 28, 1999·The Journal of Biological Chemistry·T C SungM A Frohman
Jul 30, 1999·Biochimica Et Biophysica Acta·K E MeierK M Ella
Jul 30, 1999·Biochimica Et Biophysica Acta·M LiscovitchX Tang
Apr 1, 2000·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Apr 5, 2000·The Journal of Biological Chemistry·S J WalkerH A Brown
Apr 15, 2000·The Journal of Biological Chemistry·C LeeS G Rhee
Jun 15, 2000·The Journal of Biological Chemistry·A N ProninJ L Benovic
Jun 30, 2000·Biochemical and Biophysical Research Communications·L XuD A Foster
May 25, 2001·The Journal of Biological Chemistry·S LeeS H Ryu
Jan 28, 2003·Biochemical Society Transactions·N T KtistakisJ M Sugars
Feb 14, 2004·The Journal of Biological Chemistry·Songhai ChenHeidi E Hamm
Mar 20, 2004·The Journal of Biological Chemistry·Jun-Song Chen, John H Exton
May 1, 2004·The Journal of Biological Chemistry·Juliana S Popova, Mark M Rasenick
Nov 17, 2004·The Journal of Biological Chemistry·Kirsti HillLen R Stephens
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·F Gregory BuchananJohn H Exton
Mar 22, 2005·Nature Neuroscience·Trillium BlackmerThomas F J Martin

❮ Previous
Next ❯

Citations

May 20, 2008·Cellular and Molecular Life Sciences : CMLS·A V Smrcka
Jan 25, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Paschal A Oude WeerninkMartina Schmidt
Sep 23, 2011·Chemical Reviews·Paige E SelvyH Alex Brown
Jul 14, 2010·TheScientificWorldJournal·Julian Gomez-Cambronero
Feb 24, 2010·Molecular and Cellular Biology·Karen M HenkelsJulian Gomez-Cambronero
Aug 21, 2013·Pharmacological Reports : PR·Marek KrzystanekJanusz K Rybakowski
Nov 18, 2008·Platelets·M VorlandH Holmsen
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·Y S KolesnikovV S Kravets
Feb 18, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Jul 6, 2014·Membranes·Lars-Ove BrandenburgThomas Koch
Sep 5, 2014·The Plant Journal : for Cell and Molecular Biology·Timothy E Gookin, Sarah M Assmann
Sep 24, 2014·Pharmacological Reviews·Ronald C BruntzH Alex Brown
Jun 27, 2020·Antioxidants & Redox Signaling·Nickolas AuclairEmile Levy
Jul 28, 2020·Journal of Bioenergetics and Biomembranes·Wojciech SzlasaJulita Kulbacka
Dec 13, 2019·Progress in Lipid Research·M I McDermottV A Bankaitis
Jan 27, 2021·Advances in Biological Regulation·Forrest Z BowlingMichael V Airola
Oct 24, 2006·Biochimica Et Biophysica Acta·Paschal A Oude WeerninkMartina Schmidt
Apr 4, 2009·Biochimica Et Biophysica Acta·Yasunori KanahoHiroshi Hasegawa
Mar 17, 2009·Biochimica Et Biophysica Acta·Johanna Mansfeld, Renate Ulbrich-Hofmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.