Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism

Neuron
V TropepeD van der Kooy

Abstract

Little is known about how neural stem cells are formed initially during development. We investigated whether a default mechanism of neural specification could regulate acquisition of neural stem cell identity directly from embryonic stem (ES) cells. ES cells cultured in defined, low-density conditions readily acquire a neural identity. We characterize a novel primitive neural stem cell as a component of neural lineage specification that is negatively regulated by TGFbeta-related signaling. Primitive neural stem cells have distinct growth factor requirements, express neural precursor markers, generate neurons and glia in vitro, and have neural and non-neural lineage potential in vivo. These results are consistent with a default mechanism for neural fate specification and support a model whereby definitive neural stem cell formation is preceded by a primitive neural stem cell stage during neural lineage commitment.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Dec 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·H Grunz, L Tacke
Jul 1, 1989·Developmental Biology·S M Sato, T D Sargent
Aug 1, 1989·Developmental Biology·S F Godsave, J M Slack
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Jul 27, 1995·Nature·P A Wilson, A Hemmati-Brivanlou
Apr 1, 1995·Developmental Biology·G BainD I Gottlieb
Apr 22, 1994·Cell·A Hemmati-Brivanlou, D A Melton
Oct 29, 1993·Science·T M LambR M Harland
Jan 28, 1994·Cell·T KishimotoS Akira
Jan 10, 1996·Developmental Biology·M George-WeinsteinH Weintraub
Feb 7, 1997·Cell·S J MorrisonD J Anderson
Jan 1, 1997·Annual Review of Neuroscience·A Hemmati-Brivanlou, D Melton
Apr 1, 1997·Mechanisms of Development·A FainsodM Blum
Aug 1, 1997·Trends in Neurosciences·M F Mehler, J A Kessler
Dec 29, 1998·Mechanisms of Development·A K HadjantonakisA Nagy
Apr 20, 1999·Developmental Biology·J J PearceJ Rossant
May 20, 1999·The Anatomical Record·K S O'Shea
Aug 10, 1999·Journal of Neurobiology·M F FinleyJ E Huettner
Feb 5, 2000·Cell·J L Wrana
Feb 26, 2000·Science·D van der Kooy, S Weiss
Jun 2, 2000·Science·D L ClarkeJ Frisén
May 1, 1997·Development Genes and Evolution·P A Hoodless, A Hemmati-Brivanlou
Sep 1, 1933·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·C H Waddington, G A Schmidt

❮ Previous
Next ❯

Citations

Jan 13, 2004·Neurotoxicity Research·Mary B NewmanPaul R Sanberg
Jul 26, 2011·Cellular and Molecular Life Sciences : CMLS·Huisheng Liu, Su-Chun Zhang
Oct 17, 2012·Cellular and Molecular Life Sciences : CMLS·Michele BertacchiFederico Cremisi
Aug 17, 2004·Cell and Tissue Research·Laurent RoybonJia-Yi Li
Feb 8, 2008·Bulletin of Experimental Biology and Medicine·V S RepinG T Sukhikh
Feb 22, 2011·Neurochemical Research·Jun Aruga, Katsuhiko Mikoshiba
May 27, 2011·In Vitro Cellular & Developmental Biology. Animal·Punam NagvenkarDeepa Bhartiya
Feb 16, 2010·Journal of Molecular Neuroscience : MN·Rajendran SanalkumarJackson James
Jan 22, 2004·Archives of Medical Research·José-Manuel BaizabalLuis Covarrubias
Jun 1, 2005·Biochemical and Biophysical Research Communications·Jeonghoon HeoSnorri S Thorgeirsson
Oct 1, 2013·Genomics, Proteomics & Bioinformatics·Guangjun XiQi-Long Ying
Mar 31, 2004·Journal of the Neurological Sciences·Shunmei ChibaNoboru Suzuki
Apr 9, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Mary B Newman, Roy A E Bakay
Aug 19, 2003·Developmental Biology·Noboru SatoAli H Brivanlou
Nov 19, 2003·Developmental Biology·David A F LoebelPatrick P L Tam
Feb 13, 2003·Journal of Neuroscience Methods·Ignacio Vicario, Thomas Schimmang
Nov 24, 2001·Trends in Neurosciences·H Q Xian, D I Gottlieb
Feb 20, 2003·Trends in Neurosciences·Raewyn M Seaberg, Derek van der Kooy
May 28, 2003·Neuroscience Research·Takashi NakayamaNobuo Inoue
May 29, 2002·Brain Research Bulletin·E Arenas
Sep 5, 2001·Current Opinion in Genetics & Development·J Rathjen, P D Rathjen
Mar 20, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Thorsten Gorba, Timothy E Allsopp
Sep 2, 2003·Seminars in Cell & Developmental Biology·Hideyuki Okano
Jul 4, 2009·Acta Pharmacologica Sinica·Hao-Ran Qian, Yi Yang
Mar 14, 2009·Cell Death and Differentiation·R BajpaiA V Terskikh
Dec 31, 2008·Cell Research·Tetsuro Watabe, Kohei Miyazono
Apr 5, 2012·Molecular Psychiatry·K J BrennandF H Gage
Feb 3, 2005·Nature Biotechnology·Xue-Jun LiSu-Chun Zhang
Jan 14, 2003·Nature Biotechnology·Qi-Long YingAustin Smith
Feb 8, 2005·Nature Neuroscience·Kiichi WatanabeYoshiki Sasai
Aug 2, 2007·Nature Reviews. Drug Discovery·Colin W Pouton, John M Haynes
Dec 15, 2007·Nature Reviews. Drug Discovery·Irina KlimanskayaRobert Lanza
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K S O'Shea
Dec 7, 2006·Reproduction, Fertility, and Development·Steven L SticeSoojung Shin
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takafumi WatayaYoshiki Sasai
Aug 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Magda ForsbergJonas Frisén
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dhananjay YellajoshyulaKristen L Kroll
Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wenlin LiSheng Ding
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zheng WeiLynne M Angerer
Feb 29, 2008·Molecular & Cellular Proteomics : MCP·Anastasia K YocumPhilip C Andrews
Feb 24, 2004·Journal of Hematotherapy & Stem Cell Research·Su-Chun Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.