Direct observation of ligand rebinding pathways in hemoglobin using femtosecond mid-IR spectroscopy

The Journal of Physical Chemistry. B
Seongheun KimManho Lim

Abstract

The dynamics of NO rebinding in hemoglobin (Hb) was directly observed using femtosecond mid-IR spectroscopy after photodeligation of NO from HbNO in D(2)O at 283 K. Time-resolved spectra of bound NO appeared to have a single feature peaked at 1616 cm(-1) but were much better described by two Gaussians with equal intensities but different rebinding kinetics, where the feature at 1617 cm(-1) rebinds faster than the one at 1614 cm(-1). It is possible that the two bands each correspond to one of two subunit constituents of the tetrameric Hb. Transient absorption spectra of photodeligated NO revealed three evolving bands near 1858 cm(-1) and their red-shifted replicas. The red-shifted replicas arise from photodeligated NO in the vibrationally excited v = 1 state. More than 10% of the NO was dissociated into the vibrationally excited v = 1 state when photolyzed by a 580 nm pulse. The three absorption bands for the deligated NO could be attributed to three NO sites in or near the heme pocket. The kinetics of the three transient bands for the deligated NO, as well as the recovery of the bound NO population, was most consistent with a kinetics scheme that incorporates time-dependent rebinding from one site that rapidly equilibrates with...Continue Reading

References

Dec 2, 1975·Biochemistry·R H AustinI C Gunsalus
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P A AnfinrudR M Hochstrasser
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E R HenryR M Hochstrasser
May 9, 1987·Biophysical Chemistry·A AnsariT B Sauke
Jan 5, 1983·Journal of Molecular Biology·P A CorneliusA W Steele
May 25, 1983·Journal of Molecular Biology·E R HenryW A Eaton
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J O AlbenK T Yue
Jan 27, 1995·Journal of Molecular Biology·M L QuillinH Li
Oct 27, 1994·Nature·I SchlichtingR M Sweet
Oct 1, 1993·Biophysical Journal·J R MourantR D Young
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M LimP A Anfinrud
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K KuczeraM Karplus
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·H HartmannF Parak
Mar 1, 1997·Nature Structural Biology·M LimP A Anfinrud
Apr 11, 2002·Chemical Reviews·Jens K S Møller, Leif H Skibsted
Jul 20, 2002·Biophysical Chemistry·Markus MeuwlyMartin Karplus
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jan M KrieglG Ulrich Nienhaus
Jun 21, 2003·Science·Friedrich SchottePhilip A Anfinrud
Sep 29, 2004·Journal of Structural Biology·Friedrich SchottePhilip A Anfinrud
Oct 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gerhard HummerPhilip A Anfinrud
Dec 8, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·David R Nutt, Markus Meuwly
Apr 21, 2005·Journal of the American Chemical Society·Seongheun Kim, Manho Lim
Dec 6, 2005·Biophysical Journal·David R Nutt, Markus Meuwly
Dec 29, 2007·Biochemistry·Karin NienhausG Ulrich Nienhaus
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sergei G KruglikMichel Negrerie

❮ Previous
Next ❯

Citations

Jan 28, 2016·Physical Chemistry Chemical Physics : PCCP·Taegon LeeManho Lim
Mar 21, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jaeheung ParkManho Lim
Feb 26, 2013·The Journal of Physical Chemistry. B·Jaeheung ParkManho Lim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.