Direct observation of phenylalanine orientations in statherin bound to hydroxyapatite surfaces.

Journal of the American Chemical Society
Tobias WeidnerDavid G Castner

Abstract

Extracellular biomineralization proteins such as salivary statherin control the growth of hydroxyapatite (HAP), the principal component of teeth and bones. Despite the important role that statherin plays in the regulation of hard tissue formation in humans, the surface recognition mechanisms involved are poorly understood. The protein-surface interaction likely involves very specific contacts between the surface atoms and the key protein side chains. This study demonstrates for the first time the power of combining near-edge X-ray absorption fine structure (NEXAFS) spectroscopy with element labeling to quantify the orientation of individual side chains. In this work, the 15 amino acid N-terminal binding domain of statherin has been adsorbed onto HAP surfaces, and the orientations of phenylalanine rings F7 and F14 have been determined using NEXAFS analysis and fluorine labels at individual phenylalanine sites. The NEXAFS-derived phenylalanine tilt angles have been verified with sum frequency generation spectroscopy.

References

Aug 24, 1979·Calcified Tissue International·E C MorenoD I Hay
May 13, 2004·Journal of the American Chemical Society·Piotr Cyganik, Manfred Buck
Mar 17, 2005·The Biochemical Journal·Gordon B ProctorPeter Wilde
Mar 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jie WangZhan Chen
Apr 2, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hua WangShaoyi Jiang
Jun 30, 2005·Journal of the American Chemical Society·James M GibsonGary P Drobny
Feb 24, 2006·The Journal of Physical Chemistry. B·Yan ZubavichusMichael Zharnikov
Jul 13, 2006·The Journal of Physical Chemistry. a·Yan ZubavichusMichael Zharnikov
Aug 23, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaosong LiuF J Himpsel
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gil GoobesGary P Drobny
Jan 24, 2007·Analytical and Bioanalytical Chemistry·Kenichi Ataka, Joachim Heberle
Mar 30, 2007·Biochemistry·Rivka GoobesPatrick S Stayton
Aug 1, 2007·The Journal of Physical Chemistry. B·Yan ZubavichusMichael Zharnikov
Oct 13, 2007·Journal of the American Chemical Society·Kosta MakrodimitrisJeffrey J Gray
Nov 7, 2007·Nucleic Acids Research·Eldon L UlrichJohn L Markley
Oct 22, 2008·Chemical Reviews·Mark Hildebrand
Aug 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Moise NdaoGary P Drobny
Sep 22, 2009·Journal of the American Chemical Society·Nicholas F BreenGary P Drobny
Apr 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Loren BaughDavid G Castner
Jul 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobias WeidnerDavid G Castner
Aug 10, 2010·Physical Chemistry Chemical Physics : PCCP·Frederick ChesneauMichael Zharnikov
May 4, 2011·Journal of the American Chemical Society·Li FuElsa C Y Yan
May 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Pei YangZhan Chen
Jan 24, 2012·Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B·J E BaioD G Castner
Jul 1, 2010·Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B·J E BaioDavid G Castner

❮ Previous
Next ❯

Citations

Dec 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Elisa BoaniniAdriana Bigi
Dec 15, 2015·Journal of the Royal Society, Interface·Joe E BaioTobias Weidner
Sep 4, 2015·Journal of the American Chemical Society·Denise K SchachTobias Weidner
Dec 4, 2014·Solid State Nuclear Magnetic Resonance·Wendy J Shaw
Oct 19, 2017·Physical Chemistry Chemical Physics : PCCP·Michael A DonovanTobias Weidner
Feb 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinyuan XuJianshu Li
Jun 4, 2013·Physical Chemistry Chemical Physics : PCCP·Tobias Weidner, David G Castner
Jun 30, 2018·Topics in Catalysis·Patrik K JohanssonDavid G Castner
Jul 26, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fan ZhangJianshu Li
May 13, 2021·Annual Review of Analytical Chemistry·Tobias Weidner, David G Castner
Feb 11, 2020·ACS Applied Materials & Interfaces·Lars SchmüserTobias Weidner
Jul 12, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Joshua JasenskyZhan Chen
Apr 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph H LubinJeffrey J Gray

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

© 2021 Meta ULC. All rights reserved