Direct on-the-spot detection of SARS-CoV-2 in patients

Experimental Biology and Medicine
Nadav Ben-AssaNaama Geva-Zatorsky

Abstract

Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Feb 6, 2015·BioTechniques·Nathan A TannerThomas C Evans
Feb 24, 2018·Journal of Virological Methods·Kelly A CurtisS Michele Owen
Apr 5, 2020·Journal of Clinical Microbiology·Yi-Wei TangCharles W Stratton
Apr 16, 2020·The New England Journal of Medicine·Matthew Meselson
Jun 21, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sally Cheuk Ying WongDavid Christopher Lung

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Citations

Jan 27, 2021·Microbial Biotechnology·Monika JaníkováPeter Celec
Nov 7, 2020·Experimental Biology and Medicine·Oraphan MayuramartSunchai Payungporn
Feb 18, 2021·Talanta·Jiaqi BuShian Zhong
Jul 6, 2021·Frontiers in Bioengineering and Biotechnology·Mohammad HosseinifardHamed Golmohammadi
Jun 30, 2021·Scientific Reports·Noah B ToppingsDylan R Pillai
Aug 28, 2021·Diagnostics·Gerson Shigeru KobayashiMaria Rita Passos-Bueno

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Methods Mentioned

BETA
PCR

Software Mentioned

LAMP
RT

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